Test Overview
Test Methodology

Immunofixation

Test Usage

To detect clonal light chains. Useful in characterizing the species of protein in cases of proteinuria. Techniques can identify intact monoclonal protein and free light chains (Bence Jones protein). Of value in the study of monoclonal gammopathies and lymphoproliferative diseases, including lymphoma, multiple myeloma and Waldenstrom’s macroglobulinemia. Urine immunofixation is essential in amyloidosis or if a monoclonal band is present on urine protein electrophoresis.

Reference Range *

Negative for Bence Jones protein (no clonal free light chains identified). Pathologist interpretation of results provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

Urine proteins are most concentrated in first am voided samples. Immunofixation is qualitative.

Test Details
Days Set Up
Monday, Wednesday, Thursday
Analytic Time

48 hours

Soft Order Code
BJS
MiChart Code
Bence Jones Protein, Random Urine
Synonyms
  • Immunoelectrophoresis (IEP), Urine
  • Immunofixation (IFIX), Urine
  • Kappa Light Chains, Urine
  • Lambda Light Chains, Urine
  • Light Chains, Urine
  • Myeloma Proteins, Urine
Laboratory
Chemical Pathology
Section
Immunopathology
Specimen Requirements
Collection Instructions

Collect first morning void urine specimen (preferred), preservative free. A 24 hour or random urine, preservative free, is also acceptable. Refrigerate up to 1 week.

Normal Volume
10 mL urine
Minimum Volume
5 mL urine
Additional Information

Test includes pathologist interpretation of results billed as a separate additional charge. This test is not available without interpretation.

Billing
CPT Code
86335
Fee Code
21926
Pro Fee CPT
86335-26
Pro Fee Code
86335
LOINC
55900-5
NY State Approved
No