Test Overview
Test Methodology

Fluorescence In Situ Hybridization (FISH)

Test Usage

This test detects rearrangements involving the BCL6 gene region (3q27) via fluorescence in situ hybridization (FISH) in formalin-fixed, paraffin-embedded tissue specimens. FISH is performed using a dual-color break-apart probe (Abbott Molecular) to qualitatively assess for the presence of BCL6 translocations that occur in B-cell lymphomas. Testing for BCL6 gene rearrangement may aid in the diagnosis and classification of these tumors.

Reference Range *

Interpretive report provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

This test will detect rearrangements involving BCL6, but it will not identify the translocation partner. In addition, this test may fail to detect a translocation in a sample with a low neoplastic burden.

Test Details
Days Set Up
Monday - Thursday
Analytic Time

3-10 days

Soft Order Code
MBC6
MiChart Code
BCL6 (3q27) Rearrangement by FISH
Synonyms
  • MBC6
  • MLABEL
  • BCL6BAP
  • BCL6 (3q27) Rearrangement FISH
Laboratory
Molecular Diagnostics
Section
Molecular Diagnostics
Specimen Requirements
Collection

Submit a formalin-fixed, paraffin block of tissue; store at room temperature.

Collection Instructions

A formalin-fixed, paraffin-embedded tissue block containing sufficient neoplastic cells is preferred. Unstained slides (3, 4-micron slides) with associated H&E stained slide are also acceptable. Decalcified tissue or other fixatives will be accepted and the assay attempted, however these may result in failed testing due to degraded nucleic acid. Both blocks and slides should be stored at room temperature.

Normal Volume
Formalin-fixed, paraffin-embedded tissue
Additional Information

By ordering this test the clinician acknowledges that informed consent has been obtained from the patient as required by applicable state or federal laws and the ordering clinician has authorization from the patient permitting MLabs to report the test results to the ordering clinician. Test includes pathologist interpretation of results billed as a separate additional charge. This test is not available without interpretation.

Billing
CPT Code
88377-TC
Fee Code
NA039
Pro Fee CPT
88377-26
NY State Approved
No