Test Overview
Test Methodology

Flow cytometry

Test Usage

Screening for common variable immunodeficiency and hyper-IgM syndromes, assessing B-cell subset reconstitution after stem cell or bone marrow transplant, or assessing response to B-cell-depleting immunotherapy
This test is not indicated for the evaluation of lymphoproliferative disorders (eg, leukemia, lymphoma, multiple myeloma).

Reference Range *

Interpretive report provided

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday - Friday
Analytic Time

4 - 5 days

Soft Order Code
Reference Laboratory
Special Testing
Specimen Requirements
Collection Instructions

Collect specimen in 2 EDTA tubes. Maintain one at room temp and one at refrigerated temp. Send to Specimen Processing with only this patient in each bag as to not get them mixed up.
Specimens must be received in the laboratory at Mayo on a weekday or by 4 p.m. on Friday. Collect Monday -Thursday early morning until noon, and send to SP with a stat courier.

Special Handling

Specimens must be received at Mayo weekdays and by 4 p.m. on Friday. It is recommended that specimens arrive within 24 hours of collection.

Two separate EDTA whole blood specimens are required: 1 refrigerated and 1 at ambient transport temperature.

Normal Volume
6 mL whole blood x2; maintain one tube at room temp and one tube at refrigerated temp
Minimum Volume
5 mL x2; maintain one tube at room temp and one tube at refrigerated temp
Storage Temperature
One tube ambient, one tube refrigerated
Rejection Criteria
Not received within 48 hrs, collected on a Friday-Monday, specimens not maintained at room AND refrigerated temperatures
Additional Information

Test performed by Mayo Clinic Laboratories

CPT Code
86355-B cells, total count, 86357-Natural killer (NK) cells, total count, 86359-T cells, total count, 86360-Absolute CD4/CD8 count with ratio, 86356 x7 - Mononuclear cell antigen, quantitative