Test Overview
Test Methodology

Anti-IIa clottable assay

Test Usage

Used for monitoring argatroban therapy.

Reference Range *

The concentration in a normal individual is zero. The therapeutic range for argatroban is 0.4 - 1.2 mcg/mL.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Daily, 24 hours
Analytic Time

2 hours

Soft Order Code
MiChart Code
Argatroban Level, Anti-IIa Inhibitor Assay
  • Argatroban
  • Ecarin Clotting Time
  • Anti-IIa Inhibitor Assay
STAT Availability

STAT requests for this test will be performed on a STAT basis (supervisory staff approval is not required).

Specimen Requirements
Collection Instructions

Collect specimen in a blue top (citrate 3.2%) tube. Mix by inversion. Specimen should arrive at lab within 4 hours of collection; transport at room temperature. Alternatively, centrifuge, aliquot plasma into a plastic vial, and freeze the specimen within 4 hours of collection. Transport frozen specimen on dry ice. Collection of the blood through lines that have been previously flushed with heparin should be avoided. If the blood must be drawn through a VAD (vascular access device), the line should be flushed with 5 mL of saline and the first 5 mL of blood or six dead space volumes of the VAD discarded.

Not to be used for patients on dabigatran therapy.
Normal Volume
Full 2.7mL tube or 1mL plasma aliquot
Minimum Volume
Full 1.8mL tube or 1mL plasma aliquot
Storage Temperature
Room temperature for whole blood or frozen aliquot of citrated plasma.
Rejection Criteria
Specimens will be rejected if not properly filled, clotted, grossly hemolyzed, contaminated with heparin or received outside acceptable time limits.
Additional Information

Argatroban is a direct thrombin inhibitor used for patients who cannot be treated with Heparin. The initial half-life of Argatroban is between 39-51 minutes and is cleared by the liver. Upon discontinuation of therapy, anticoagulant parameters return to baseline within 2-4 hours.

CPT Code
Fee Code