Test Overview
Test Methodology

Multiplex Flow Immunoassay

Test Usage

The ANA screen test detects autoantibodies to 11 clinically relevant antibodies (dsDNA, SS-A, SS-B, Sm, RNP, SmRNP, Scl-70, Jo-1, Centromere B, Ribosomal P, and Chromatin). A negative result means that the patient’s serum shows no reactivity for these common antibodies associated with connective tissue diseases. See also: Extractable Nuclear Antibodies.

Reference Range *

Negative

Test Details
Days Set Up
Monday - Friday
Analytic Time

24 hours

Soft Order Code
ANA/ANA2
Synonyms
  • Nuclear Antibody Screen
  • ANA Screen
  • Connective Tissue Disorder Screen
  • LE Prep Replacement Assay
  • Lupus Test Replacement Assay
  • ANA
  • ANTI-NUCLEAR ANTIBODY SCREEN
Laboratory
Chemical Pathology
Section
Immunopathology
Specimen Requirements
Collection Instructions

Collect specimen in SST tube. Centrifuge, aliquot serum into a plastic vial and refrigerate up to 7 days. Please note if ANA by Multiplexed Immunoassay with Reflex to IFA Titer and Pattern is desired (ANA2).

Alternate Specimen
Red top tube.
Plasma, EDTA or heparinized, is acceptable.
Yellow Top Tube
Normal Volume
0.5 mL serum
Minimum Volume
0.25 mL serum
Additional Information

The ANA by Multiplex Flow Immunoassay has a lower positive rate in patients without connective tissue diseases and eliminates some of the very weak positives (titer 1:80) often seen by IFA that have no detectable specific antibodies. If ANA by Multiplexed Immunoassay with Reflex to IFA Titer and Pattern (ANA2) is requested and the screen is positive, a titer will be performed at an additional charge. If the Hep-2 ANA titer is 1:80 or greater with a homogeneous, speckled, nucleolar, or atypical nuclear matrix pattern in children less than 18 years of age, the more specific tissue ANA will be performed at an additional charge. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated.

Billing
CPT Code
86038
Fee Code
30950
LOINC
ANA 8061-4, ANA2 47383-5