Michigan Medicine: Chemiluminescent Immunoassay
Mayo Clinic Laboratories: Enzyme-Linked Immunosorbant Assay (ELISA)
Females age 12-45 yrs: 0.8 - 9.5 ng/mL, age >45 yrs: <1.0 ng/mL.
AMH performed in the Chemical Pathology Laboratory is measured by the Roche e411 chemiluminescent immunoassay. AMH concentrations in females peak at 25 years and decline with age until menopause. In patients with polycystic ovarian syndrome, AMH concentrations may be 2 to 5 fold higher than age appropriate reference levels. If results are inconsistent with the clinical picture, the Chemical Pathology laboratory at 936-6702.
Testing for all male and female pediatric (age <12 yrs) will be sent to Mayo Clinic Laboratories: Males: age <2 yrs: 14 - 466 ng/mL; age 2-12 yrs: 7.4 - 243 ng/mL; age >12 yrs: 0.7 - 19 ng/mL. Females: age <2 yrs: <4.7 ng/mL; age 2-12 yrs: <8.8 ng/mL.
- Mullerian Inhibiting Substance (MIS)
- Anti Mullerian Hormone
- Antimullerian Hormone, Serum
Collect specimen in an SST tube. Centrifuge, aliquot serum into a plastic vial and refrigerate. Specimen should be collected on days 2-4 of the menstrual cycle. Samples should not be drawn from patients receiving therapy with high biotin doses (>5 mg/day) until at least 8 hours following the last biotin administration.
Only female patients 12 years and older testing will be performed at Michigan Medicine. All male and female patients <12 years testing will be performed by Mayo Clinic Laboratories.
With the Roche methodology (Chemical Pathology), interference was observed at biotin concentrations above 30 ng/mL. Samples should not be drawn from patients receiving therapy with high biotin doses (>5 mg/day) until at least 8 hours following the last biotin administration.