Test Overview
Test Methodology

Indirect Fluorescent Antibody (IFA), human neutrophil substrate
Multiplex Flow Immunoassay

Test Usage

Neutrophil cytoplasmic antibody testing is useful in the evaluation of patients with vasculitis. GPA is a disease which results in a necrotizing granulomatous vasculitis in the upper and lower respiratory tract as well as in the kidney. Autoantibodies against neutrophil cytoplasmic antigens have been found in these patients. These antibodies have also been observed in microscopic polyarteritis, and other types of vasculitis.

Reference Range *

Negative. Pathologists interpretation of results provided. This panel includes neutrophil cytoplasmic antibody testing by indirect immunofluorescence (IFA), anti-proteinase 3 and anti-myeloperoxidase. Two common patterns have been recognized. One pattern is a finely granular staining of cytoplasmic granules; C-Neutrophil Cytoplasmic autoantibodies (C-NCA). The most common antibody that accounts for this pattern is anti-proteinase 3. C-NCA has been described in patients with Granulomatosis with Polyangiitis (GPA), polyarteritis nodosa and some types of crescentic glomerulonephritis. The second pattern shows a strong perinuclear fluorescence but little or no staining in the cytoplasm. This pattern has been attributed to anti-myeloperoxidase. The perinuclear NCA pattern (P-NCA) is associated with vasculitis, but is not as specific for GPA as the cytoplasmic (C-NCA) pattern.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday, Thursday
Analytic Time

8 hours

Soft Order Code
NCABP
MiChart Code
ANCA (Neutrophil Cytoplasmic Ab) Panel w/ Anti-MPO and PR3
Synonyms
    Laboratory
    Chemical Pathology
    Section
    Immunopathology
    Specimen Requirements
    Collection Instructions

    Collect specimen in SST tube. Centrifuge, aliquot serum into a plastic vial and freeze.

    Alternate Specimen
    Red Top Tube
    Normal Volume
    0.5 mL serum
    Minimum Volume
    0.5 mL serum
    Rejection Criteria
    Sample received in Immunology >48 hours and not frozen.
    Additional Information

    If the IFA screen is positive, a titer will be performed at an additional charge. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. Test includes pathologist interpretation of results billed as a separate additional charge. This test is not available without interpretation.

    Billing
    CPT Code
    86036, 83516 x2
    Fee Code
    KA041 NCAB, 21595 PR3, 21594 MYELO
    Pro Fee CPT
    86036-26 NCAB Screen, 86037-26 NCAB Titer
    Reflex CPT
    86037
    Reflex Fee Code
    KA042
    LOINC
    17353-4 NCAB,14277-8 NCABT, 46267-1 PR3N, 63310-7 PR3I, 46266-3 MYLON, 29643-4 MYLOI
    NY State Approved
    No