Test Overview
Test Methodology

Liquid Chromatography - Tandem Mass Spectrometry (LC-MS/MS)

Test Usage

Monitor therapeutic drug levels; monitor patient compliance; evaluate amitriptyline toxicity.

Reference Range *

Therapeutic: 120-250 ng/mL; Toxic: > 500 ng/mL (sum of Amitriptyline and Nortriptyline).

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

Phenothiazines and other tricyclic antidepressants and their metabolites may interfere when administered concomitantly.

Test Details
Days Set Up
Thursday
Analytic Time

4 hours

Soft Order Code
AMTRP
MiChart Code
Amitriptyline and Nortriptyline Level
Synonyms
  • Elavil
  • Endep
  • Etrafon
  • Triavil
  • Tricyclics, Amitriptyline and Nortriptyline
  • AMITRIPTYLINE/NORTRIPTYLINE LV
  • AMITRIPTYLINE RESULT
  • NORTRIPTYLINE RESULT
  • AMITRIPTYLINE/NORTRIPTYLINE
  • AMIT
  • NORT2
  • AMITRIPTYLINE
  • NORTRIPTYLINE
Laboratory
Chemical Pathology
Section
Drug Analysis
Specimen Requirements
Collection Instructions

Collect blood in a red top or green top tube; do not use SST or PST tube. Centrifuge, aliquot serum or plasma into plastic vial as soon as possible and refrigerate.

Alternate Specimen
Laveder top (EDTA plasma) [11/09]
Rejection Criteria
SST or PST tube not acceptable.
Yellow Top Tube
Red Top Tube
Normal Volume
1 mL serum or plasma
Minimum Volume
0.7 mL serum or plasma
Additional Information

Test includes Amitriptyline and its active metabolite, Nortriptyline.

Billing
CPT Code
80335
Fee Code
KA006
LOINC
Z107-3
Resources