Test Overview
Test Methodology

Liquid Chromatography - Tandem Mass Spectrometry (LC-MS/MS)

Test Usage

Monitor therapeutic drug levels; monitor patient compliance; evaluate amitriptyline toxicity.

Reference Range *

Amitriptyline and Nortriptyline: Therapeutic: 80 - 200 ng/mL. Nortriptyline only: Therapeutic: 70 - 170 ng/mL. Therapeutic ranges are for specimens drawn at trough (ie, immediately before next scheduled dose). Levels may be elevated in non-trough specimens.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Tuesday, Thursday, Sunday
Analytic Time

2 - 5 days

Soft Order Code
MAMTR
MiChart Code
Amitriptyline and Nortriptyline Level
Synonyms
  • Elavil
  • Endep
  • Etrafon
  • Triavil
  • Tricyclics, Amitriptyline and Nortriptyline
Laboratory
Sendout
Reference Laboratory
Mayo AMTRP
Section
Special Testing
Specimen Requirements
Collection Instructions

Collect specimen in a red top tube immediately before next scheduled dose (minimum 12 hours after last dose); do not use SST tube. Centrifuge, aliquot serum into plastic vial within 2 hours of collection, and refrigerate.

Normal Volume
1 mL serum
Minimum Volume
0.25 mL serum
Rejection Criteria
SST or PST tube not acceptable.
Additional Information

Test includes Amitriptyline and its active metabolite, Nortriptyline. Test sent to Mayo Clinic Laboratories.

Billing
CPT Code
80335
Fee Code
AB137
LOINC
43106-4
NY State Approved
No