Test Overview
Test Methodology

Enzymatic End point/Spectrofluorometric

Test Usage

Confirmation of a diagnosis of aminolevulinic acid dehydratase deficiency porphyria (ADP).

Reference Range *

> or = to 4.0 nmol/L/sec (normal), 3.5 - 3.9 nmol/L/sec (indeterminate), <3.5 nmol/L/sec (diminished). Reference ranges have not been established for patients who are <16 years of age.

Test Details
Days Set Up
Tuesday, Thursday
Analytic Time

2 - 4 days

Soft Order Code
ALADR
MiChart Code
ALA Dehydratase, RBC
Synonyms
  • Aminolevulinic Acid Dehydratase (ALA-D), Washed Erythrocytes
Laboratory
Sendout
Reference Laboratory
Mayo ALADW
Section
Special Testing
Specimen Requirements
Collection

Patient should abstain from alcohol for 24 hours prior to specimen collection. Collect blood in a green top tube. Place on wet ice immediately. Process as follows:
a. Immediately place specimen on wet ice.
b. Transfer entire specimen to a 12-mL graduated centrifuge tube.
c. Centrifuge specimen for 10 minutes at 2,000 rpm.
d. Record volume of packed cells and the total volume of the specimen.
e. Discard supernatant plasma.
f. Wash packed erythrocytes 2 times by resuspension of at least an equal amount of cold 0.9% saline, mix, and centrifuge for 5 minutes at 2,000 rpm, discarding supernatant after each washing.
g. Resuspend packed cells to the original total volume with 0.9% saline. Invert specimen gently to mix.
Volume of packed cells and total volume of erythrocyte suspension (red cells + saline) are required and must be sent with specimen. Include a list of medications the patient is currently taking.

Special Handling

Place on ice immediately following collection. See below for processing instuctions.

Normal Volume
3 mL washed erythrocyte suspension
Minimum Volume
1 mL washed erythrocyte suspension
Additional Information

Test sent to Mayo Medical Laboratories.

Billing
CPT Code
82657
Fee Code
20404
NY State Approved
No