Determining the tissue source of increased Alkaline Phosphatase activity in serum and differentiating between liver and bone sources of elevated Alkaline Phosphatase.
Age and sex specific reference ranges provided with report.
2 - 5 days
- ALK Phosphatase Fractionation
- Alkaline Phosphatase Isoenzyme, Bone
- Alkaline Phosphatase Isoenzyme, Liver
- Fractionated Alkaline Phosphatase
- Regan Isoenzymes
- Alkaline Phosphatase Isoenzyme
- 5'Nucleotidase, Serum
- Alkaline Phosphatase Isoenzyme, Intestine
Collect blood in a red top or SST tube. Centrifuge, aliquot serum into two plastic vials (0.5 mL each), and freeze. Hemolyzed specimens are not acceptable.
Test includes Total Alkaline Phosphatase and Bone, Liver, and Intestine Isoenzymes. Alkaline phosphatase (ALP) has at least nine recognized isoenzymes. Each has varying properties of heat stability, chemical inhibition, and electrophoretic mobility. They include fast (pre-liver), liver, bone, placental, Regan, Nagao, renal, intestinal, and PA. Of primary interest in the laboratory is distinguishing bone from liver. Of secondary interest is the presence of the more heat-stable "tumor" isoenzymes: Regan, Nagao, and renal. Test sent to Mayo Medical Laboratories.