Test Overview
Test Methodology

Electrophoresis, Densitometry

Test Usage

Determining the tissue source of increased Alkaline Phosphatase activity in serum and differentiating between liver and bone sources of elevated Alkaline Phosphatase.

Reference Range *

Age and sex specific reference ranges provided with report.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday - Friday
Analytic Time

2 - 5 days

Soft Order Code
MiChart Code
Alkaline phosphatase, isoenzymes
  • ALK Phosphatase Fractionation
  • Alkaline Phosphatase Isoenzyme, Bone
  • Alkaline Phosphatase Isoenzyme, Liver
  • Fractionated Alkaline Phosphatase
  • Regan Isoenzymes
  • Alkaline Phosphatase Isoenzyme
  • 5NT
  • 5'Nucleotidase, Serum
  • Alkaline Phosphatase Isoenzyme, Intestine
Reference Laboratory
Mayo ALKI (89503)
Special Testing
Specimen Requirements
Collection Instructions

Collect blood in a red top or SST tube. Centrifuge, aliquot serum into two plastic vials (0.5 mL each), and freeze. Hemolyzed specimens are not acceptable.

Normal Volume
1 mL serum (two 0.5 mL aliquots)
Minimum Volume
0.5 mL serum (two 0.25 mL aliquots)
Additional Information

Test includes Total Alkaline Phosphatase and Bone, Liver, and Intestine Isoenzymes. Alkaline phosphatase (ALP) has at least nine recognized isoenzymes. Each has varying properties of heat stability, chemical inhibition, and electrophoretic mobility. They include fast (pre-liver), liver, bone, placental, Regan, Nagao, renal, intestinal, and PA. Of primary interest in the laboratory is distinguishing bone from liver. Of secondary interest is the presence of the more heat-stable "tumor" isoenzymes: Regan, Nagao, and renal. Test sent to Mayo Medical Laboratories.

CPT Code
Fee Code