Isolate and identify potentially pathogenic organisms
Normal eye flora
If the specimen is refrigerated, Neisseria gonorrhoeae, Hemophilus influenzae and other fastidious organisms may not be isolated, even though they are present in the specimen. Normal eye skin flora includes diphtheroids, alpha Streptococcus, gamma Streptococcus, Micrococcus sp, Neisseria sp (not N. gonorrhoeae or N. meningitidis), Bacillus sp. (not B. anthracis or B. cereus), Hemophilus sp. (not H. influenzae) and coagulase negative staphylococcus. If present, these organisms will be reported as 'skin flora present'.
Preliminary results are reported in 18-24 hours. The health care provider will be notified when culture becomes positive with possible Pseudomonas, Neisseria gonorrhoeae, Bacillus cereus, Group A beta streptococcus, Staphylococcus aureus, yeast, or molds. Negative cultures are reported after 3 days.
- Conjunctiva Culture, Aerobic
- Cornea, Routine Culture
- Culture, Conjunctiva, Aerobic
- Eye, Routine Culture
- Routine Culture, Conjunctiva
Cleanse skin around eye with mild antiseptic and collect eye swab, corneal scrapings, conjunctiva, or donor cornea. Swab: pass swab moistened with preservative-free saline 2 times over lower conjunctiva, avoiding eyelid border and lashes. Scrapings: use local anesthetic and platinum spatula. Scraping should be done by ophthalmologist. (OD = right eye, OS = left eye). If orbital cellulitis is suspected, write "cellulitis" on the requisition.
Includes direct smear and culture. On corneal specimens, all organisms are identified, unless the specimen appears contaminated with skin flora. Test includes susceptibility testing of potentially pathogenic organism(s) at an additional charge unless specifically declined.