Optical detection of clot formation in the Dilute Russell's Viper Venom Time (DRVVT).
Activated Protein C Resistance is associated with a point mutation in the Factor V gene. This mutation slows the inactivation of factor Va by APC causing a hypercoagulable state.
Ratio greater than or equal to 1.8
Initial oral anticoagulant treatment before stabilization and levels of Protein C below 50% may give low APCR ratios.
- APC Resistance
- Factor V Leiden Mutation Screen
- Protein C Resistance, Activated
- ACTIVATED PROTEIN C RESISTANCE
- Factor 5 Leiden Mutation Screen
Collect specimen in a blue top (citrate 3.2%) tube. Mix by inversion. Specimen should arrive at lab within 3 hours of collection; transport at room temperature. Alternatively, centrifuge, aliquot plasma into a plastic vial, and freeze the specimen within 4 hours of collection. Transport frozen specimen on dry ice. Collection of the blood through lines that have been previously flushed with heparin should be avoided. If the blood must be drawn through a VAD (vascular access device), the line should be flushed with 5 mL of saline and the first 5 mL of blood or six dead space volumes of the VAD discarded.
Samples with positive or borderline results should be confirmed by genetic analysis as Factor V Leiden is
a genetic mutation.