Test Overview
Test Methodology

Real-Time Polymerase Chain Reaction (PCR)/TaqMan DNA Probe Hybridization

Test Usage

Aid in the diagnosis of amebic keratitis in conjunction with clinical findings.

Reference Range *


* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday - Saturday
Analytic Time

3 - 4 days

Soft Order Code
MiChart Code
Acanthamoeba / Naegleria Exam
  • ACAN
  • Cornea, Acanthamoeba Exam
  • Cornea, Naegleria Exam
  • Eye, Acanthamoeba Exam
  • Eye, Naegleria Exam
  • Naegleria Exam
  • Parasite Exam, Acanthamoeba
  • Parasite Exam, Naegleria
  • Amebic Keratitis (AK)
  • ACSRC - Specimen Source
Reference Laboratory
Special Testing
Specimen Requirements
Collection Instructions

The preferred specimen for Acanthamoeba PCR from an ocular source is corneal scraping or biopsy. Fluorescein dye may interfere with testing and should not be used prior to collection. Submit only one of the following specimens: 1) fresh ocular tissue, ocular or corneal scrapings, or contact lenses in a sterile container with 1 mL of sterile saline, minimal essential media (MEM), or viral transport media 2) contact lens solution in a sterile container, 3) formalin-fixed paraffin-embedded (FFPE) ocular tissue in a sterile container. Refrigerate.

Alternate Specimen
Other specimens: FFPE ocular tissue, scrapings on a swab, contact lenses, contact lens case without the contacts
Normal Volume
1 mL fluid or 5-10 mm tissue, corneal scrapings is the preferred tissue for this test, in sterile saline solution or VTM
Additional Information

Test sent to Mayo Medical Laboratories.

CPT Code