Test Overview
Real-time quaking-induced conversion assay (RT-QuIC)
Quantitative Enzyme-Linked Immunosorbant Assay (ELISA)
Evaluation of patients with rapidly progressive dementia to establish the diagnosis of CJD.
RT-QuIC: Negative
Total Tau (ELISA): 0-1149 pg/mL
14-3-3 GAMMA (ELISA): 173-1999 AU/mL
* Reference ranges may change over time. Please refer to the original patient report when evaluating results.
Measurement of 14-3-3 protein in CSF should not be relied upon exclusively to establish the diagnosis of Creutzfeldt-Jakob disease (CJD). Increased concentrations in CSF have been described in various diseases that affect the CNS.
Test Details
7 days for CSF testing, 3 days for a biopsy, 30 days for a genetic workup
- 14-3-3 Protein, Spinal Fluid
- Creutzfeldt-Jakob Disease
- CJD
- Prion
- 1433
Specimen Requirements
The first 2 mL of CSF that flows from the tap should be discarded. Collect 2mL of CSF (1 mL min) in CSF collection tubes, avoiding a bloody tap. Freeze specimen immediately at -20 degrees. Ship to Specimen Processing frozen. Test can also be performed on frozen or fixed brain tissue, blood, or urine. Tissue can be from autopsy or biopsy. Please note "Suspected CJD" on all specimens and requisition.
A completed National Prion Disease Pathology Surveillance Center (NPDPSC) test request form available online at https://case.edu/medicine/pathology/sites/default/files/2024-06/Test%20… must be submitted with the specimen.
Additional Information
Test sent to National Prion Survelliance Center, Cleveland, OH. UMHS users may refer to the Infection Control "Hospital Precautions for Creutzfeldt-Jakob Disease Policy" at http://www.med.umich.edu/i/ice/policies/pdfs/cjd/cjd.pdf for more information.