Test Overview
Test Methodology

Real-time quaking-induced conversion assay (RT-QuIC)

Test Usage

Evaluation of patients with rapidly progressive dementia to establish the diagnosis of CJD.

Reference Range *


* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

Measurement of 14-3-3 protein in CSF should not be relied upon exclusively to establish the diagnosis of Creutzfeldt-Jakob disease (CJD). Increased concentrations in CSF have been described in various diseases that affect the CNS.

Test Details
Analytic Time

Turnaround time is approximately two weeks from receipt of the samples for autopsies and approximately three days for biopsies.

Soft Order Code
MiChart Code
National Prion 14-3-3 Testing
  • 14-3-3 Protein, Spinal Fluid
  • Creutzfeldt-Jakob Disease
  • CJD
  • Prion
  • 1433
Reference Laboratory
National Prion Survelliance Center
Special Testing
Specimen Requirements
Collection Instructions

Collect CSF and place in a sterile tube or container. Freeze. Test can also be performed on frozen or fixed brain tissue, blood, or urine. Tissue can be from autopsy or biopsy. Please note "Suspected CJD" on all specimens and requisition.

A completed National Prion Disease Pathology Surveillance Center (NPDPSC) test request form available online at https://case.edu/medicine/pathology/sites/case.edu.pathology/files/2021… must be submitted with the specimen.

Normal Volume
1 mL CSF
Minimum Volume
0.5 mL CSF
Additional Information

Test sent to National Prion Survelliance Center, Cleveland, OH. UMHS users may refer to the Infection Control "Hospital Precautions for Creutzfeldt-Jakob Disease Policy" at http://www.med.umich.edu/i/ice/policies/pdfs/cjd/cjd.pdf for more information.

CPT Code