NEW TEST
IGH::MYC t(8;14)(q24;q32) Translocation by FISH
Order Code: MIHMY
Fee Code: NA123 (CPT 88377-TC, 88377-26)
Effective November 8, 2023, the MLabs Molecular Diagnostics Laboratory offers IGH::MYC t(8;14)(q24;q32) Translocation by FISH as a new available test. This dual-fusion FISH test may identify MYC rearrangements not detected by MYC break-apart FISH and may, therefore, be used in conjunction with the MYC (8q24) Rearrangement by FISH test. The IGH::MYC t(8;14)(q24;q32) rearrangement occurs in most Burkitt lymphomas and a subset of large B-cell lymphomas including high-grade B-cell lymphoma with MYC and BCL2 rearrangements.
Test Usage: This test detects the IGH::MYC t(8;14)(q24;q32) rearrangement using fluorescence in situ hybridization (FISH). FISH is performed with fluorescent probes directed at IGH (14q32), MYC (8q24) and the centromere of chromosome 8. The IGH::MYC t(8;14)(q24;q32) rearrangement occurs in most Burkitt lymphomas and a subset of large B-cell lymphomas including high-grade B-cell lymphoma with MYC and BCL2 rearrangements. Importantly, this test may identify MYC rearrangements not detected by MYC break-apart FISH and may, therefore, be used in conjunction with the MYC (8q24) Rearrangement by FISH test.
Test Limitations: A negative result does not exclude a MYC rearrangement involving translocation partners other than IGH. This test may fail to detect a translocation in a sample with a low neoplastic burden.
Collection Instructions: A formalin-fixed, paraffin-embedded tissue block (containing sufficient neoplastic cells) is preferred. Unstained slides (3 slides cut at 4-microns) with associated H&E-stained slide are also acceptable. Decalcified tissue or tissues with other fixatives will be accepted and the assay attempted; however, these specimens may result in failed testing due to degraded nucleic acid. Both blocks and slides should be stored at room temperature.