Test Overview
Test Methodology

IgM Antibody Capture Enzyme-Linked Immunosorbent Assay (MAC-ELISA)

Test Usage

Screening for the presence of IgM-class antibodies to Zika virus in symptomatic pregnant women with either travel to a Zika virus endemic region or who have had sexual exposure to an individual with Zika virus.

Reference Range *

Negative

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

This assay is a screening test for IgM-class antibodies to Zika virus. A presumptive positive result by this assay is not diagnostic for Zika virus infection and requires confirmatory testing by plaque reduction neutralization testing (PRNT) performed at the CDC or a CDC-designated laboratory. Confirmatory testing by a molecular assay for detection of Zika virus RNA may also be considered.
This assay should be used in patients presenting at least 2 weeks postsymptom onset or last possible exposure to Zika virus. Reverse transcription-polymerase chain reaction (RT-PCR) for Zika virus RNA detection should be ordered in patients with less than 2 weeks of symptoms or postexposure.
A single negative result by the Zika IgM ELISA should not be used to rule-out infection as the specimen may have been collected prior to the development of detectable antibodies.

Test Details
Days Set Up
Monday, Wednesday, Friday
Analytic Time

2 - 5 days

Soft Order Code
PNZIK
Laboratory
Sendout
Reference Laboratory
Mayo PNZIK
Section
Special Testing
Test Updated
Update Type
Replacement Test
Specimen Requirements
Collection Instructions

Collect specimen in SST or red top tube. Centrifuge, aliquot serum into a plastic screw capped vial, and freeze.

Yellow Top Tube
Normal Volume
2.5 mL serum
Minimum Volume
2 mL serum
Additional Information

Due to similar clinical presentation and cross reactivity, testing for IgM-class antibodies to dengue virus, concurrently with Zika virus IgM testing, is recommended (Dengue Virus Antibody/Antigen Panel, Serum). Test sent to Mayo Medical Laboratories.

Billing
CPT Code
86794
Fee Code
AA378
NY State Approved
No
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