Test Overview

Test Methodology

Fluorescence Polarization Immunoassay (FPIA)

Test Usage

Monitoring of drug therapy

Reference Range*

Therapeutic: Peak: 20 - 40 mcg/mL, Trough: 5 - 20 mcg/mL; Critical: Peak/Random values: >60 mcg/mL; Trough values: >20.1 mcg/mL.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details

Days Test Performed

Daily, 24 hrs

Analytic Time

2 hours

Soft Order Code


MiChart Code

Vancomycin, Trough


Chemical Pathology

STAT Availability

STAT requests for this test will be performed on a STAT basis (supervisory staff approval is not required).

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Specimen Requirements

Offsite Collection Instructions

Collect blood in an SST tube. Centrifuge, aliquot serum into a plastic vial and refrigerate. For maximum clinical significance, usual sampling times are Peak: approximately 30 minutes after the end of a 1 hour I.V. infusion or Trough: immediately before next dose is given. Indicate whether specimen is Peak, Trough, or Random level.


Yellow Top Tube

Normal Volume

0.5 mL serum

Minimum Volume

0.25 mL serum

Billing Information

Fee Codes

CPT Code



Trough 4092-3; Peak 4090-7; Random 4091-5

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