Days Set Up
Collect blood or bone marrow in a lavender top tube. Refrigerate and send intact blood or bone marrow specimen within 48 hours of collection. Fresh tissue (preferably 0.5 cm3, sent in RPMI) and fresh aspirates or body fluids are acceptable. Refrigerate an
Analytic Time

7-14 days

MiChart Code
TP53 Mutation in Malignancy
Soft Order Code
TP53M

Test Updated:

Synonyms
 

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Test Overview

Test Methodology

Next-Generation Sequencing

Test Usage

TP53 encodes a tumor suppressor protein that normally responds to diverse cellular stresses to induce cell cycle arrest, apoptosis, senescence, DNA repair or changes in metabolism. Somatic, loss of function mutations in TP53 are one of the most frequent in cancer and result in loss of the anti-proliferative and tumor suppressive effects of the p53 protein. In some malignancies, such as acute myeloid leukemia (AML) and chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), TP53 mutations are associated with an adverse prognosis resulting in the use of more aggressive treatment. Somatic mutations can also be associated with age-related clonal hematopoiesis in the absence of an overt hematologic malignancy. Germline TP53 mutations are associated with Li-Fraumeni Syndrome – a familial predisposition to a variety of malignancies including sarcomas, breast carcinoma, gliomas and adrenocortical carcinomas.

This DNA test is performed by targeted next-generation sequencing (NGS) and will detect mutations within the sequenced regions of TP53 exons 2 - 11 (NM_000546.5). See the table below for the specific regions sequenced by this assay. The reference genome assembly used for alignment and variant calling is hg19 (GRCh37).

Reference Range

Interpretive Report Provided
*Reference ranges may change over time. Please refer to the original patient report when evaluating results.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Specimen Requirements

Collection Onsite

Collect blood or bone marrow in a lavender top tube. Refrigerate and send intact blood or bone marrow specimen within 48 hours of collection. Fresh tissue (preferably 0.5 cm3, sent in RPMI) and fresh aspirates or body fluids are acceptable. Refrigerate and send, preferably within 24 hours. Frozen tissue specimens – preferably frozen with 1 hour of collection – may also be sent frozen on dry ice. Fresh cell suspensions in RPMI should be refrigerated and sent, preferably within 48 hours. Frozen cell suspensions – preferably frozen with 1 hour of collection – may also be sent frozen on dry ice. Fixed cytogenetic cell suspensions or pellets in Carnoy’s fixative. Refrigerate and send. For formalin-fixed, paraffin-embedded tissue, a block containing an area with a high percentage of neoplastic cells is preferred. Unstained, UNBAKED slides (5-8, 10-micron slides; 10-15 if few neoplastic cells are present) with associated H&E stained slide are also acceptable. Decalcified tissue or other fixatives will be accepted and the assay attempted; however, these may result in failed testing due to degraded nucleic acid. Both blocks and slides should be stored at room temperature.

Collection Offsite

Collect blood or bone marrow in a lavender top tube. Refrigerate and send intact blood or bone marrow specimen within 48 hours of collection. Fresh tissue (preferably 0.5 cm3, sent in RPMI) and fresh aspirates or body fluids are acceptable. Refrigerate and send, preferably within 24 hours. Frozen tissue specimens – preferably frozen with 1 hour of collection – may also be sent frozen on dry ice. Fresh cell suspensions in RPMI should be refrigerated and sent, preferably within 48 hours. Frozen cell suspensions – preferably frozen with 1 hour of collection – may also be sent frozen on dry ice. Fixed cytogenetic cell suspensions or pellets in Carnoy’s fixative. Refrigerate and send. For formalin-fixed, paraffin-embedded tissue, a block containing an area with a high percentage of neoplastic cells is preferred. Unstained, UNBAKED slides (5-8, 10-micron slides; 10-15 if few neoplastic cells are present) with associated H&E stained slide are also acceptable. Decalcified tissue or other fixatives will be accepted and the assay attempted; however, these may result in failed testing due to degraded nucleic acid. Both blocks and slides should be stored at room temperature.

Normal Volume
5 mL EDTA (lavender) whole blood; 3 mL EDTA (lavender) bone marrow; Fresh/frozen tissue and fresh aspirates or body fluids; Fresh/frozen cells in RPMI; Fixed cytogenetic cell suspensions or pellets in Carnoy’s fixative; Formalin-fixed, paraffin-embedded tissue.
Minimum Volume
 

Billing Information

CPT Code
81405
Pro Fee Code
G0452-26
LOINC
 

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Our High Standard

Quality that sets us apart

As the reference laboratory division of Michigan Medicine's Department of Pathology, MLabs shares the institution's commitment to applying established quality principles to clinical laboratory testing. Like other large organizations in complex, consequential fields, we rely on an established approach to monitor quality throughout the testing process.