Test will include additional crossmatch testing if indicated. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. The Flow Crossmatch uses indirect immunofluorescence to detect human alloantibodies bound to target lymphocytes. IgG antibodies appear to be most associated with rejection; therefore a fluorophore cojugated anti human IgG reagent is used to detect the alloantibody binding. A second and third fluorescent parameter is used to delineate the desired cell population (T or B cell). Interpretation of the Flow Crossmatch is done by comparing the fluorescence intensity of the donor’s cells mixed with the patient's serum to the fluorescence intensity of the same donor when mixed with a known negative control serum. This fluorescent value is then converted mathematically into Median Channel Shift (MCS) or Molecules of Equivalent Soluble Fluorochrome (MESF).