Test Overview
Test Methodology

Flow Cytometry

Test Usage

A flow crossmatch between the recipient and donor may need to be repeated due to unexpected results, low donor lymphocyte counts, or other unforeseen problems. The lymphocyte crossmatch assesses the level of circulating antibodies in the potential recipient that are directed against HLA Class I and/or Class II molecules of the donor.

Reference Range *

Interpretive report provided.

Test Details
Days Set Up
Monday - Thursday, 8:00am - 4:30pm; Friday 8:00am - 10:30am
Analytic Time

5 days

Soft Order Code
HLCDC
Synonyms
  • HLA REPORT
  • FLRPT
  • Living Donor Repeat XM
  • HLFXM
  • TISSUE TYPING
  • TTYPE
  • HLA Crossmatch Flow
  • Flow Crossmatch, Donor, Repeat
  • Kidney Transplant Repeat Flow Crossmatch
Laboratory
Histocompatibility/Immunogenetics
Section
Histocompatibility
Specimen Requirements
Collection

Collect specimen in sufficient yellow top (ACD) solution A tubes. Send intact specimen at room temperature. Do not refrigerate or freeze. Testing must be performed within 14 days prior to transplant.

Alternate Specimen
Green top tube (sodium or lithium heparin) acceptable. (5/99)
Normal Volume
40 mL ACD (solution A) whole blood
Minimum Volume
30 mL ACD (solution A) whole blood
Additional Information

Test will include additional crossmatch testing if indicated. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. The Flow Crossmatch uses indirect immunofluorescence to detect human alloantibodies bound to target lymphocytes. IgG antibodies appear to be most associated with rejection; therefore a fluorophore cojugated anti human IgG reagent is used to detect the alloantibody binding. A second and third fluorescent parameter is used to delineate the desired cell population (T or B cell). Interpretation of the Flow Crossmatch is done by comparing the fluorescence intensity of the donor’s cells mixed with the patient's serum to the fluorescence intensity of the same donor when mixed with a known negative control serum. This fluorescent value is then converted mathematically into Median Channel Shift (MCS) or Molecules of Equivalent Soluble Fluorochrome (MESF).

Billing
CPT Code
86825
Fee Code
37953
Reflex CPT
86826
Reflex Fee Code
10383
NY State Approved
No