Test Overview

Test Methodology

Molecular Typing (SSO)

Test Usage

At the UMHS Histocompatibility Laboratory potential recipients are typed for HLA-A, B, C, DRB1,3,4,5, DQB1, DQA1 loci by medium/low resolution molecular methods (Antigen level). When a potential related or unrelated living donor is considered for transplant, the HLA typing of the donor is performed to evaluate compatibility. Donors are typed for the same HLA loci and for DPB1 in case of anti-HLA DPB1 detected on the recipient serum. The lymphocyte crossmatch assesses the level of circulating antibodies in the potential recipient that are directed against HLA Class I and/or Class II molecules of the donor.

Reference Range*

Interpretive report provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details

Days Test Performed

Monday - Thursday, 8:00am - 4:30pm; Friday 8:00am - 10:30am

Analytic Time

5 days

Soft Order Code




HLA Class I Low Resolution
HLA Class II Low Resolution
New Kidney Donor
Kidney Transplant Donor
Flow Crossmatch, Kidney Donor
HLA Crossmatch Flow
HLA Typing Class I and II

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Specimen Requirements


ACD Solution A

Normal Volume

10 mL ACD (solution A) whole blood

Minimum Volume

10 mL ACD (solution A) whole blood

Additional Information

Test includes HLA Typing Class I & II.

Billing Information

Fee Codes

CPT Code

81372, 81376

Reflex Fee Code


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