Test Overview
Test Methodology

Solid Phase Antibody Screening

Test Usage

The antibodies directed to the possible donor or an already transplanted organ are known as Donor Specific Antibodies (DSA). These DSA are associated with an increased risk of graft loss and/or rejection. The damage produced by the recognition of the HLA antigens on the surface of the graft endothelium by the DSA result in the rejection process known as Antibody Mediated Rejection (AMR).
The high specificity and sensitivity of the current solid phase single antigen assay allow us the detection of low-level of the precise DSA. The detection of DSA in pre-transplant evaluation is an excellent tool for risk assessment and crossmatch prediction (Virtual Crossmatch and CPRA). The routine monitoring of DSA in the post-transplant setting assist the transplant team in the characterization of the rejection process and the treatment decisions.

Test Details
Days Set Up
Monday - Thursday, 8:00am - 4:30pm; Friday 8:00am - 2:00pm
Analytic Time

STAT: 48 hours; Routine: 5 days

Soft Order Code
HLAS
Synonyms
  • HLAS
  • HLA Antibody Specificity Class II
  • HLA Antibody Specificity Class I
  • HLA REPORT
  • TISSUE TYPING
  • TTYPE
  • DSA
  • Donor Specific Ab Testing
  • Donor Specific Antibody (DSA)
  • Class I & II SAB Antibody Screening
Laboratory
Histocompatibility/Immunogenetics
Section
Histocompatibility
Specimen Requirements
Collection

Collect specimen in a red top (preferred) or SST tube. Send intact specimen at room temperature. Do not refrigerate or freeze. Specify whether testing is STAT or routine.

Yellow Top Tube
Normal Volume
5 mL serum
Minimum Volume
2 mL serum
Billing
CPT Code
86832, 86833
Fee Code
37962, 37963
NY State Approved
No