Solid Phase Assay
Complement cascade activation by Donor Specific Antibodies (DSA) is needed for the pathogenesis of the Antibody Mediated Rejection process. Not every IgG subclass binds complement with the same intensity; IgG1 and IgG3 are the two subclasses with higher capability to bind and activate complement. IgG2 activates complement with much lower intensity and IgG4 does not activate complement. The relative concentration of the different subclasses of IgG is in direct relation with the pathogenicity of the polyclonal DSA in circulation.To measure the complement binding capability of the anti HLA-antibodies we use a C1q binding assay. The binding of this molecule is measure utilizing the same high specificity and sensitivity solid phase single antigen platform that is used for the total IgG detection.This tool helps the transplant team in the risk assessment for these patients with DSA and also provides valuable information of the efficacy of the antibody removal therapies.
Days Test Performed
Monday - Friday, 8:00am - 4:30pm
HLA Antibody Specificity Class II
HLA Antibody Specificity Class I
C1q Binding HLA Antibody
Looking to Order a Test?
We’ve provided helpful links to make ordering easy.
All specimens should be accompanied by a requisition.
Learn about how to properly label and where to ship specimens.
MLabs provides all the supplies necessary for the collection of specimens.
Visit our provider FAQ and learn about common questions to ordering tests.
5 mL serum
3 mL serum