Test Overview

Test Methodology

Solid Phase Assay

Test Usage

Complement cascade activation by Donor Specific Antibodies (DSA) is needed for the pathogenesis of the Antibody Mediated Rejection process. Not every IgG subclass binds complement with the same intensity; IgG1 and IgG3 are the two subclasses with higher capability to bind and activate complement. IgG2 activates complement with much lower intensity and IgG4 does not activate complement. The relative concentration of the different subclasses of IgG is in direct relation with the pathogenicity of the polyclonal DSA in circulation.To measure the complement binding capability of the anti HLA-antibodies we use a C1q binding assay. The binding of this molecule is measure utilizing the same high specificity and sensitivity solid phase single antigen platform that is used for the total IgG detection.This tool helps the transplant team in the risk assessment for these patients with DSA and also provides valuable information of the efficacy of the antibody removal therapies.

Test Details

Days Test Performed

Monday - Friday, 8:00am - 4:30pm

Analytic Time

5 days



HLA Antibody Specificity Class II
HLA Antibody Specificity Class I
C1q Binding HLA Antibody

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Specimen Requirements

Normal Volume

5 mL serum

Minimum Volume

3 mL serum

Billing Information

Fee Codes

CPT Code

86832, 86833

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