2 - 4 days
Protoporphyrins, Fractionation, Washed Erythrocytes
Looking to order a test?
We’ve provided helpful links to make ordering easy.Find a Requisition
All specimens should be accompanied by a requisition.Submitting Specimens
Learn about how to properly label and where to ship specimens.Order Kits and Supplies
MLabs provides all the supplies necessary for the collection of specimens.Test FAQ
Visit our provider FAQ and learn about common questions to ordering tests.
High-Performance Liquid Chromatography (HPLC) with Fluorescence Detection
Establishing a biochemical diagnosis of erythropoietic protoporphyria and X-linked dominant protoporphyria.
Free Protoporphyrin: <20 mcg/dL packed cells; Zinc-Complexed Protoporphyrin: <60 mcg/dL packed cells.
* Reference ranges may change over time. Please refer to the original patient report when evaluating results.
Patient should abstain from alcohol for 24 hours and be off medications for 1 week prior to specimen collection. If it is clinically inappropriate to discontinue medications, forward a list of medications with the specimen. Collect blood in a green top tube from a fasting patient (12 - 14 hour fast). Place on wet ice immediately. Process as follows:
a. Transfer entire specimen to a 12-mL graduated centrifuge tube.
b. Centrifuge specimen for 10 minutes at 2,000 rpm.
c. Record volume of packed cells and the total volume of the specimen.
d. Discard supernatant plasma.
e. Wash packed erythrocytes 2 times by resuspension of at least an equal amount of cold 0.9% saline, mix, and centrifuge for 5 minutes at 2,000 rpm, discarding supernatant after each washing.
f. Resuspend packed cells to the original total volume with 0.9% saline. Invert specimen gently to mix.
g. Transfer washed erythrocytes into a plastic vial and freeze.
Volume of packed cells and total volume of erythrocyte suspension (red cells + saline) are required and must be sent with specimen.