Days Set Up
Monday, Wednesday, Friday
Analytic Time

2 - 4 days

MiChart Code
Protoporphyrins, Fractionation, RBC
Soft Order Code
PPFWE

Test Updated:

Synonyms

Protoporphyrins, Fractionation, Washed Erythrocytes

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All specimens should be accompanied by a requisition.

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Test Overview

Test Methodology

High-Performance Liquid Chromatography (HPLC) with Fluorescence Detection

Test Usage

Establishing a biochemical diagnosis of erythropoietic protoporphyria and X-linked dominant protoporphyria.

Reference Range

Free Protoporphyrin: <20 mcg/dL packed cells; Zinc-Complexed Protoporphyrin: <60 mcg/dL packed cells.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Specimen Requirements

Collection

Patient should abstain from alcohol for 24 hours and be off medications for 1 week prior to specimen collection. If it is clinically inappropriate to discontinue medications, forward a list of medications with the specimen. Collect blood in a green top tube from a fasting patient (12 - 14 hour fast). Place on wet ice immediately. Process as follows:
a. Transfer entire specimen to a 12-mL graduated centrifuge tube.
b. Centrifuge specimen for 10 minutes at 2,000 rpm.
c. Record volume of packed cells and the total volume of the specimen.
d. Discard supernatant plasma.
e. Wash packed erythrocytes 2 times by resuspension of at least an equal amount of cold 0.9% saline, mix, and centrifuge for 5 minutes at 2,000 rpm, discarding supernatant after each washing.
f. Resuspend packed cells to the original total volume with 0.9% saline. Invert specimen gently to mix.
g. Transfer washed erythrocytes into a plastic vial and freeze.
Volume of packed cells and total volume of erythrocyte suspension (red cells + saline) are required and must be sent with specimen.

Normal Volume
3 mL washed erythrocyte suspension
Minimum Volume
1 mL washed erythrocyte suspension

Billing Information

CPT Code
82542
Pro Fee Code
 
LOINC
 

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Our High Standard

Quality that sets us apart

As the reference laboratory division of Michigan Medicine's Department of Pathology, MLabs shares the institution's commitment to applying established quality principles to clinical laboratory testing. Like other large organizations in complex, consequential fields, we rely on an established approach to monitor quality throughout the testing process.