Days Set Up
Tuesday, Thursday
Analytic Time

3 - 5 days

MiChart Code
Porphobilinogen Deaminase (PBGD), Washed Erythrocytes
Soft Order Code
PBGDW

Test Updated:

Synonyms

Porghobilinogen Deaminase (PBG-D), Washed Erythrocytes

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All specimens should be accompanied by a requisition.

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Test Overview

Test Methodology

Enzymatic End point/Spectrofluorometric

Test Usage

Confirmation of a diagnosis of acute intermittent porphyria (AIP).

Reference Range

> or = 7.0 nmol/L sec; 6.0 - 6.9 nmol/L sec (indeterminate); <6.0 nmol/L/sec (diminished). Reference ranges have not been established for patients who are <16 years of age.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Specimen Requirements

Collection

Patient should abstain from alcohol for 24 hours and be off medications for 1 week prior to specimen collection. If it is clinically inappropriate to discontinue medications, forward a list of medications with the specimen. Collect blood in a green top tube from a fasting patient (12 - 14 hour fast). Place on wet ice immediately. Process as follows:
a. Immediately place specimen on wet ice.
b. Transfer entire specimen to a 12-mL graduated centrifuge tube.
c. Centrifuge specimen for 10 minutes at 2,000 rpm.
d. Record volume of packed cells and the total volume of the specimen.
e. Discard supernatant plasma.
f. Wash packed erythrocytes 2 times by resuspension of at least an equal amount of cold 0.9% saline, mix, and centrifuge for 5 minutes at 2,000 rpm, discarding supernatant after each washing.
g. Resuspend packed cells to the original total volume with 0.9% saline. Invert specimen gently to mix.
H.Transfer washed erythrocytes into a plastic vial and freeze.
Volume of packed cells and total volume of erythrocyte suspension (red cells + saline) are required and must be sent with specimen.

Normal Volume
3 mL washed erythrocyte suspension
Minimum Volume
1 mL washed erythrocyte suspension

Billing Information

CPT Code
82657
Pro Fee Code
 
LOINC
 

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Our High Standard

Quality that sets us apart

As the reference laboratory division of Michigan Medicine's Department of Pathology, MLabs shares the institution's commitment to applying established quality principles to clinical laboratory testing. Like other large organizations in complex, consequential fields, we rely on an established approach to monitor quality throughout the testing process.