Abbott i-Stat - two-site enzyme-linked immunosorbent assay (ELISA)
Performed at authorized locations by point of care operators. Troponin is the contraction regulating protein complex of striated muscle and consists of three distinct proteins: Troponin-I, Troponin-C, and Troponin-T. Troponin-I subunit exists in three isoforms. two in skeletal muscle and one in cardiac muscle. The cardia form is 40% dissimilar from skeletal forms and thus can be used as an aid in diagnosis of Acute myocardial infarction (AMI).
Cardiac Troponin-I is measurable in serum within 4 to 6 hours after the onset of an AMI, with peak concentrations being reached in approximately 12-18 hours after infarction. Unlike other cardiac markers (CK-MB, myoglobin) Troponin-I levels may remain elevated in serum for several days before returning to normal.
•Clotted samples may yield falsely elevated results.
•Grossly hemolyzed samples may have falsely decreased values.
•Grossly icteric or grossly lipemic samples may affect results.
•Heterophilic antibodies in serum samples may cause interference in the immunoassay. These antibodies may be present in blood samples from individuals regularly exposed to animals or who have been treated with animal serum products.
Collect in lithium heparin syringe/tube.
•Samples in anticoagulant tubes other than lithium or sodium heparin.
•Air bubbles in sample.
•Grossly hemolyzed, lipemic, and/or icteric samples may affect test results.
For more test specific information, please refer to the test procedure found on the Michigan Medicine POC website: https://www.pathology.med.umich.edu/point-of-care