Test Overview
Test Methodology

Abbott i-Stat - two-site enzyme-linked immunosorbent assay (ELISA)

Test Usage

Performed at authorized locations by point of care operators. Troponin is the contraction regulating protein complex of striated muscle and consists of three distinct proteins: Troponin-I, Troponin-C, and Troponin-T. Troponin-I subunit exists in three isoforms. two in skeletal muscle and one in cardiac muscle. The cardia form is 40% dissimilar from skeletal forms and thus can be used as an aid in diagnosis of Acute myocardial infarction (AMI).
Cardiac Troponin-I is measurable in serum within 4 to 6 hours after the onset of an AMI, with peak concentrations being reached in approximately 12-18 hours after infarction. Unlike other cardiac markers (CK-MB, myoglobin) Troponin-I levels may remain elevated in serum for several days before returning to normal.

Reference Range *

<0.08 ng/mL

Test Limitations

•Clotted samples may yield falsely elevated results.
•Grossly hemolyzed samples may have falsely decreased values.
•Grossly icteric or grossly lipemic samples may affect results.
•Heterophilic antibodies in serum samples may cause interference in the immunoassay. These antibodies may be present in blood samples from individuals regularly exposed to animals or who have been treated with animal serum products.

Test Details
Analytic Time

10 minutes

Soft Order Code
MiChart Code
Troponin- I, Point of Care
Point of Care
Point of Care
Specimen Requirements
Collection Instructions

Collect in lithium heparin syringe/tube.

Normal Volume
3 mL whole blood
Minimum Volume
50 µL whole blood
Rejection Criteria
•Clotted samples.
•Samples in anticoagulant tubes other than lithium or sodium heparin.
•Air bubbles in sample.
•Grossly hemolyzed, lipemic, and/or icteric samples may affect test results.
Additional Information

For more test specific information, please refer to the test procedure found on the Michigan Medicine POC website: https://www.pathology.med.umich.edu/point-of-care

CPT Code
NY State Approved