Perform an attentive DRE immediately prior to specimen collection (the specimen should be collected within approximately 1 hour of DRE). Instruct patient to collect the FIRST 20 - 30 mL of voided urine following the DRE. Process the specimen as follows within 4 hours of collection (if specimen cannot be processed within 15 minutes, store refrigerated or on ice and process within 4 hours): 1) Invert specimen cup 5 times to re-suspend cells. 2) Add 2.5 mL of urine to each of two GEN-PROBE PROGENSA PCA3 Urine Specimen Transport Tubes. Do NOT puncture the foil seal on the cap of the Transport Tube. The specimen volume must fall between the two black fill lines on the Transport Tube. 3) Tightly re-cap each Urine Transport Tube and gently invert 5 times to mix; do not shake or vortex. The specimen is now a "processed" urine specimen. Refrigerate. Refrigerated specimens must be received by performing lab within 5 days of collection. For longer storage, freeze specimen at -20 degrees C and ship specimen on dry ice. Frozen specimens are stable for 90 days at -20 degrees C. Include serum PSA result and patient history with the specimen.