Test Overview

Test Methodology

Flow Crossmatch

Test Usage

This test may be diagnostically useful for post-transplant patients who appear to be undergoing humoral graft rejection but have no history of donor specific anti-HLA antibodies. This assay is also recommended for risk assessment of highly sensitized recipients previously exposed to a vascularized tissue like a previous transplant or vascular reconstruction surgeries.

Reference Range*

Interpretive report provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details

Days Test Performed

Monday - Thursday, 8:00am - 4:30pm; Friday 8:00am - 10:00am

Analytic Time

3 - 10 days

Soft Order Code




HLA Crossmatch Endothelial Precursor
Endothelial Precursor, Recip.
Endothelial Precursor, Recipient
Endothelial Precursor Flow Crossmatch, Recipient

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Specimen Requirements


Yellow Top Tube
Red Top Tube

Normal Volume

5 mL serum

Minimum Volume

3 mL serum

Additional Information

Required: "Solid Organ Transplant, Donor Cross-match Cells" must be placed on donor.

This test utilizes endothelial precursor cells to monitor the presence of non-HLA antibodies. The specificity of these antibodies remains unknown, however, some of them belong to a group of antibodies known as Anti Endothelial Cells Antibodies (AECA). This is a commercially available crossmatch kit and uses the endothelial precursor’s cells as a surrogate for the human vascular endothelial cells to detect AECA antibodies. The test is used for the detection of IgG and IgM antibodies against antigens on the surface precursor endothelial cells of the donor. These antibodies may be anti-HLA or may be due to other non-HLA antigens. It has been noted that endothelial cells are immediate targets by the host’s immune system during allograft rejection.

Billing Information

Fee Codes

CPT Code


Reflex Fee Code


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