Test Overview
Test Methodology

Flow Crossmatch

Test Usage

This test may be diagnostically useful for post-transplant patients who appear to be undergoing humoral graft rejection but have no history of donor specific anti-HLA antibodies. This assay is also recommended for risk assessment of highly sensitized recipients previously exposed to a vascularized tissue like a previous transplant or vascular reconstruction surgeries.

Reference Range *

Interpretive report provided.

Test Details
Days Set Up
Monday - Thursday, 8:00am - 4:30pm; Friday 8:00am - 10:00am
Analytic Time

3 - 10 days

Soft Order Code
HLEXM
MiChart Code
HLA Crossmatch EP
Synonyms
  • TISSUE TYPING
  • HLA REPORT
  • HLEXM
  • HLA Crossmatch Endothelial Precursor
  • TTYPE
  • ENDOD
  • Endothelial Precursor, Donor
  • Endothelial Precursor Flow Crossmatch, Donor
Laboratory
Histocompatibility/Immunogenetics
Section
Histocompatibility
Specimen Requirements
Collection

Collect specimen in sufficient yellow top (ACD) solution A tubes from donor. Send intact specimen at room temperature. Do not refrigerate or freeze. For recipient specimen please refer to Solid Organ Transplant, Endothelial Precursor Cell (Recipient).

Normal Volume
40 mL ACD (solution A) whole blood
Minimum Volume
30 mL ACD (solution A) whole blood
Additional Information

Test will include additional crossmatch testing if indicated. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. This test utilizes endothelial precursor cells to monitor the presence of non-HLA antibodies. The specificity of these antibodies remains unknown, however, some of them belong to a group of antibodies known as Anti Endothelial Cells Antibodies (AECA). This is a commercially available crossmatch kit and uses the endothelial precursor’s cells as a surrogate for the human vascular endothelial cells to detect AECA antibodies. The test is used for the detection of IgG and IgM antibodies against antigens on the surface precursor endothelial cells of the donor. These antibodies may be anti-HLA or may be due to other non-HLA antigens. It has been noted that endothelial cells are immediate targets by the host’s immune system during allograft rejection.

Billing
CPT Code
86825
Fee Code
37937
Reflex CPT
86826
Reflex Fee Code
10384
NY State Approved
No