Days Set Up
Daily
Analytic Time

2 hours

MiChart Code
HSV (Herpes Simplex Virus) Antigen
Soft Order Code
HSVD

Test Updated:

Synonyms

HSV Antigen
HSV1D
HSV2D
HSVD
Smear for Viral Antigen
Viral Antigen: Herpes simplex
HERPES SIMPLEX ANTIGEN-DFA

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Find a Requisition

All specimens should be accompanied by a requisition.

Submitting Specimens

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Order Kits and Supplies

MLabs provides all the supplies necessary for the collection of specimens.

Test FAQ

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Test Overview

Test Methodology

Direct Immunofluorescent Antibody (DFA)

Test Usage

Rapid detection of Herpes simplex infections.

Reference Range

Negative

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Specimen Requirements

Collection Onsite

The specimen type is two 3-well teflon coated slides onto which have been scraped basal cells from the base and periphery of the lesion. Using a pencil, label the frosted end of the slide with the patient's first and last name and medical record number. Carefully open the vesicle, soak up the fluid with a sterile swab place into M4 Transport Medium. Send to the laboratory for viral culture. Scrape the base and periphery of the lesion with a sharp instrument or vigorously rub with a dry swab. Gently roll the cells onto the slide, rolling each well separately. Allow the slide to air dry. The laboratory should have at least one slide with 3 wells for the test. Slides are rejected if there are not appropriate cells on the slide, if the cells are on the wrong side of the slide, if the slides are grossly contaminated, if the slides are not labeled with two patient identifiers, or if the slides are labeled with a label that covers the non-frosted area of the slide.

Collection Offsite

Special 3-well Teflon slides are available from MLabs. Using a pencil, label the frosted end of the slide with the patient's first and last names as well as a second identifier such as the patient's medical record number. One slide containing epithelial cells scraped from the base and periphery of the lesion is required. Carefully open the vesicle, soak up the fluid with a dacron or rayon swab, place into M4 transport and use for viral culture. Scrape the base and periphery of the lesion with a sharp instrument or vigorously rub with a dry swab. Gently roll the cells onto the slide, rolling each well separately. Allow the slide to air dry and refrigerate.

Normal Volume
1 slide; 2 slides are optimal
Minimum Volume
1 slide; 2 slides are optimal

Billing Information

CPT Code
87300
Pro Fee Code
 
LOINC
 

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Our High Standard

Quality that sets us apart

As the reference laboratory division of Michigan Medicine's Department of Pathology, MLabs shares the institution's commitment to applying established quality principles to clinical laboratory testing. Like other large organizations in complex, consequential fields, we rely on an established approach to monitor quality throughout the testing process.