Special 3-well Teflon slides are available from MLabs. Using a pencil, label the frosted end of the slide with the patient's first and last names as well as a second identifier such as the patient's medical record number. One slide containing epithelial cells scraped from the base and periphery of the lesion is required. Carefully open the vesicle, soak up the fluid with a dacron or rayon swab, place into M4 transport and use for viral culture. Scrape the base and periphery of the lesion with a sharp instrument or vigorously rub with a dry swab. Gently roll the cells onto the slide, rolling each well separately. Allow the slide to air dry and refrigerate.