Test Overview
Test Methodology

High Performance Liquid Chromatography (HPLC)

Reference Range *

Amphotericin B steady-state peak levels after intravenous administration: Mean adult peak level (3 mg/kg as sulfate): 2.6 mcg/mL; Mean adult peak level (4 mg/kg as sulfate): 2.9 mcg/mL; Mean adult peak level (1 mg/kg as lipid): 12.2 mcg/mL; Mean adult peak level (2.5 mg/kg as lipid): 31.4 mcg/mL; Mean adult peak level (5 mg/kg as lipid): 83.0 mcg/mL.

Test Limitations

Sulindac may interfere with this assay.

Test Details
Days Set Up
Monday, Thursday
Analytic Time

5 - 6 days

Soft Order Code
MMLR
Laboratory
Sendout
Reference Laboratory
Mayo AMPHB (80429) (Focus 51426)
Section
Special Testing
Specimen Requirements
Collection Instructions

Collect specimen in a red top; do not use SST tube. Centrifuge, aliquot serum into a plastic vial and freeze. Protect specimen from light (place in brown plastic bag or aliquot into brown tube). Collect Peak specimen 30 minutes after the end of an I.V. infusion (one hour following I.M. or oral administration) or Trough specimen immediately before next dose is given.

Special Handling

Protect specimen from light.

Rejection Criteria
SST tube not acceptable.
Yellow Top Tube
Red Top Tube
Normal Volume
2 mL serum
Minimum Volume
0.5 mL serum
Additional Information

Test sent to Mayo Medical Laboratories; performed by Focus Diagnostics.

Billing
CPT Code
80299
NY State Approved
No