Days Set Up
Monday, Thursday
Analytic Time

8 hours

MiChart Code
Anti-Neutrophilic Cytoplasmic Antibody
Soft Order Code

Test Updated:


Wegener's Granulomatosis (Vasculitis)
Antineutrophil Cytoplasmic Ab
Neutrophil Cytoplasmic Ab, TTR

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Test Overview

Test Methodology

Indirect Fluorescent Antibody (IFA), human neutrophil substrate

Test Usage

Wegener's granulomatosis is a disease which results in a necrotizing granulomatous vasculitis in the upper and lower respiratory tract as well as in the kidney. The disease has been successfully treated with immunosuppressive drugs. Recently, autoantibodies against neutrophil cytoplasmic antigens have been found in these patients. The antibody is not directed against neutrophil surface antigens. These antibodies have also been observed in microscopic polyarteritis, in systemic lupus erythematosus and in vasculitis. The antibodies correlate best with individuals having a necrotizing crescentic glomerulonephritis with or without systemic illness.

Reference Range

Negative. Pathologists interpretation of results provided. Two patterns have been recognized. True positive NCA is a finely granular pattern which stains all of the cytoplasm; C-Neutrophil Cytoplasmic autoantibodies (C-NCA) have been described in patients with Wegener's granulomatosis, polyarteritis nodosa and some types of crescentic glomerulonephritis. The second pattern shows a strong perinuclear fluorescence but little or no staining in the cytoplasm. This pattern has been attributed to anti-myeloperoxidase. The perinuclear NCA pattern (P-NCA) is associated with vasculitis, but is not as specific for Wegener's granulomatosis as the cytoplasmic (C-NCA) pattern.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Specimen Requirements

Collection Offsite

Collect specimen in SST tube. Centrifuge, aliquot serum into a plastic vial and freeze.

Yellow Top Tube
Normal Volume
0.5 mL serum
Minimum Volume
0.5 mL serum

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Quality that sets us apart

As the reference laboratory division of Michigan Medicine's Department of Pathology, MLabs shares the institution's commitment to applying established quality principles to clinical laboratory testing. Like other large organizations in complex, consequential fields, we rely on an established approach to monitor quality throughout the testing process.