Test Overview
Test Methodology

Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)

Test Usage

Detection of Influenza A and B.

Reference Range *


* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

A negative test does not rule out infection. The specimen must be taken at the appropriate time during infection and properly stored and transported; if optimum conditions are not met, the viral agents may not be detected in the presence of an infection.

Test Details
Days Set Up
Analytic Time

1 day (6 hours for UM inpatients and ED)

Soft Order Code
MiChart Code
Influenza Virus by PCR
Specimen Requirements

SWAB: Use flocked swab, dacron or rayon swab to obtain specimen and place into M4-RT transport medium. Refrigerate. NASAL WASH: Use a 1 oz rubber bulb containing 3 - 5 mL of sterile preservative-free saline and rapidly instill and aspirate the contents from a nostril in a single motion. Empty material into M4-RT transport medium (preferred) or sterile container and close cap tightly. Refrigerate. NASAL ASPIRATE: Gently insert a number 5 - 8 French pediatric-feeding tube attached to a suction strap through both nostrils into the nasopharynx. Aspirate contents into M4-RT transport medium and close cap tightly. Refrigerate. BAL FLUID: Place in sterile cup and refrigerate. M6-RT or UTM transport media is also acceptable. Deliver all specimen types to the laboratory as soon as possible. The possibility for virus isolation decreases as the length of storage time increases. Many viral agents will survive at room temperature for several hours; however, for optimum recovery of viruses, please refrigerate; do not freeze.

Alternate Specimen
M6-RT (Remel M6) or UTM (BD Universal Transport Medium) transport media is acceptable.
Normal Volume
1 nasopharyngeal swab
Additional Information

Specimen will be held for a minimum of 2 weeks to allow additional testing to be added as needed.

CPT Code
Fee Code