Days Set Up
Analytic Time

2 hours

MiChart Code
Methotrexate, 24 Hour
Soft Order Code

Test Updated:


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All specimens should be accompanied by a requisition.

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MLabs provides all the supplies necessary for the collection of specimens.

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Test Overview

Test Methodology


Test Usage

Monitor therapeutic drug level, evaluate potential toxicity

Reference Range

No precise relationship between methotrexate serum levels and antineoplastic efficacy has been established. Following a 4-6 hour intravenous methotrexate infusion with dosages ranging from 50 mg/m2 - 15 g/m2, a patient with a 24 hr serum level > 5 - 10 umol/L, a 48 hr serum level > 0.5 - 1.0 umol/L, and a 72 hr serum level > 0.2 umol/L is at an increased risk of toxicity if conventional low dose leucovorin rescue is given. In this case, high dose leucovorin rescue would be indicated.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Specimen Requirements

Collection Onsite

Collect blood in a red top or green top tube; do not use SST tube. Cerebrospinal fluid is also an acceptable specimen.

Collection Offsite

Collect blood in a red top or green top tube; do not use SST tube. Centrifuge, aliquot serum into a plastic vial, and refrigerate. Cerebrospinal fluid is also an acceptable specimen.

Yellow Top Tube
Normal Volume
0.5 mL serum, plasma, or CSF
Minimum Volume
0.25 mL serum, plasma, or CSF

Billing Information

CPT Code
Pro Fee Code

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Our High Standard

Quality that sets us apart

As the reference laboratory division of Michigan Medicine's Department of Pathology, MLabs shares the institution's commitment to applying established quality principles to clinical laboratory testing. Like other large organizations in complex, consequential fields, we rely on an established approach to monitor quality throughout the testing process.