Test Overview
Test Methodology

Direct immunofluorescence using flow cytometric techniques.

Test Usage

Confirmation of diagnosis and immunophenotype in cutaneous T-cell lymphoma. This test is used for phenotypic profiling of circulating T-cells in patients with known or suspected cutaneous T-cell lymphoma or quantitation and monitoring of aberrant T-cell populations in peripheral blood during or after treatment. This assay is not recommended for phenotypic profiling of other known or suspected T-cell lymphomas. See http://www.pathology.med.umich.edu/handbook/Tables/Flow_Cytometry_Panel… for all Flow Cytometry Panels.

Reference Range *

<a text="DERMF reference ranges.docx" href="/static/apps/handbook/Tables/DERMF reference ranges.docx">
<b>*Pediatric Reference Ranges from Journal of Allergy and Clinical Immunology, vol. 112, No. 5, 11/2003 and studies performed at Michigan Medicine Flow Cytometry Laboratory. Absolute ranges are calculated utilizing absolute lymphocyte count ranges for the same age groups provided by the hematology laboratory. </b>

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

Requires an adequate number of viable cells for analysis. Improper collection or transport may result in decreased cell viability and cancellation of testing.

Test Details
Days Set Up
Monday - Friday, 8:00am - 4:30pm; Saturday 8:00am-12:00noon
Analytic Time

8 - 48 hrs

Soft Order Code
DERMF
MiChart Code
Cutaneous T-cell Lymphoma Profile, Flow Cytometry
Synonyms
  • Cutaneous T-cell Lymphoma Pnl
  • DERMFS
  • DERMF
  • DERM
  • Sezary Cell
  • Sezary Syndrome
  • T-Cell Leukemia
  • Cutaneous T-Cell Lymphoma Panel
  • Leukemia/Lymphoma Derm Marker Panel
  • Panel 5: Cutaneous T-cell Lymphoma
Laboratory
Flow Cytometry
Section
Flow Cytometry
Specimen Requirements
Collection Instructions

The following information is required on a Hematopathology Consult requisition:
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Relevant clinical history
Clinical or morphological findings and suspicions
Date and time of collection
Phone number and after hours pager number for ordering physician
[/list]
<ul><li>
Collect blood in ACD A or B Vacutainer® tube and transport at room temperature within 12 hours of collection.
Include a copy of the patient's concurrently drawn White Blood Cell and Platelet counts, peripheral blood differential and and 2 unstained peripheral blood smears.
</ul>

Alternate Specimen
Green sodium heparin or lavender EDTA Vacutainer® tubes are acceptable but not preferred (must be received within 12 hours of collection). EDTA specimens greater than 30 hrs old cannot be processed.
Rejection Criteria
Green top tubes containing lithium heparin are not acceptable. Clotted specimens will be rejected.
Normal Volume
Whole blood: 7-10 ml
Minimum Volume
Whole blood: 1 ml added to ACD A or B tube
Additional Information

Test includes pathologist interpretation of results billed as a separate additional charge. This test is not available without interpretation. Additonal markers will be run if clinically indicated. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. For consultation, contact the MLabs Client Services Center or Flow Cytometry Laboratory at 76-39420.

Billing
CPT Code
88184, 88185 x9, 88188
Fee Code
23036, 23041 x9
Pro Fee Code
88188.1
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