Days Set Up
Monday - Friday, 8:00am - 5:00pm
Analytic Time

24 - 48 hours

MiChart Code
FNA Sample to Cytology
Soft Order Code
FNASH

Test Updated:

Synonyms

Aspiration Cytology
Needle Aspiration Cytology
FNA
Fine Needle Aspiration Biopsy
ASP
EFNA
EVALUATION OF FINE NEEDLE ASP
ASP3
FN ASPIRATION + EFNA + COLLECT
ASP2
FN ASPIRATION + EFNA
FNAS
FNA Sample to Cytology
CYTONG
Cytology, Non-Gyn

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Find a Requisition

All specimens should be accompanied by a requisition.

Submitting Specimens

Learn about how to properly label and where to ship specimens.

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MLabs provides all the supplies necessary for the collection of specimens.

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Test Overview

Test Methodology
 
Test Usage

To establish the presence of primary or metastatic malignant neoplasm.

Reference Range

Interpretive report provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Specimen Requirements

Collection Onsite

Cytopathology faculty and staff are available to assist with rapid on-site adequacy assessment and preliminary interpretation. Cytopathology faculty are also available to perform fine needle aspiration of superficial masses. Call 734-936-6799 to schedule an appointment. ASPIRATION PROCEDURE FOR SUPERFICIAL MASSES:
[list order = Y]
Cleanse the skin over the lesion with an alcohol swab. If local anesthesia is needed, use ethyl chloride spray or 1% lidocaine.
Fix the mass between your fingers to immobilize the mass during the needle passes.
Carefully poise the 23 or 25-guage needle at right angles to the surface of the skin, just touching the point of insertion. Quickly introduce the needle through the skin and advance it into the mass. When the needle has entered the mass, apply strong suction.
Apply negative pressure to the syringe and move the needle back and forth within the mass and in different directions to effect the cutting action of the needle point and retain cells in the needle hollow.
It is not necessary to see visible aspirated material in the hub, but the moment blood or any material appears in the hub, stop aspirating but do not remove the needle from the mass until pressure on the plunger is released; this will allow cells to be contained in the needle and not the syringe. If you do not release the suction, the aspirated material will enter the barrel of the syringe and may result in loss of cellular material as it is difficult to recover material from the syringe.
Place the entire sample directly in CytoLyt® solution by aspirating CytoLyt® solution through the needle into the syringe and then expelling back into the CytoLyt® container several times (see below). Samples from different anatomic sites should be placed in separate containers or slides, labeled appropriately, and submitted with separate requisitions.
Occasionally the aspirated lesion is cystic. In this case, aspirate as much fluid as possible and place the fluid into the CytoLyt® vial. Resample any residual mass following the procedure described above.
[/list] SLIDE PREPARATION AND NEEDLE RINSE: Prepare 2 smears for each pass; an adequate specimen generally consists of 3-4 separate passes. Label two glass slides with the patient's last name, first initial and second unique patient identifier such as patient's date of birth or registration number using a graphite pencil (do not use ink, wax or crayon). Leave room for laboratory staff to add accession number. Also label a CytoLyt container with the patient's last name, first initial and second unique patient identifier such as patient's date of birth or registration number. Detach the needle from the syringe, introduce air into syringe, and carefully reattach needle. Holding needle at the surface of one slide, express 1 to 2 drops of material onto the slide. Place a second slide (label side down) against the slide with expressed material. Allow the material to spread between the two slides and gently pull the slides apart by sliding them apart. Immediately fix one of the two slides with spray fixative. Allow the other slide to air dry. Label the air-dried slide "Air Dried". Rinse the needle in CytoLyt solution by aspirating the solution through the needle into the syringe and expelling back into the CytoLyt container several times. Discard the needle and syringe. Allow spray-fixed slides to dry completely and place into a slide holder. Deliver slides and CytoLyt rinse with appropriately completed requisition, including pertinent patient history immediately to Specimen Processing or to the Cytopathology Laboratory during laboratory hours. Refrigerate specimens that cannot be delivered during laboratory hours and deliver as soon as possible the following work day. Do not allow specimen to freeze.

Collection Offsite

Patients may be referred to the University of Michigan Health System for fine needle aspiration of superficial masses to be performed by Cytopathology faculty. Please call 734-936-6799 to schedule an appointment. ASPIRATION PROCEDURE FOR SUPERFICIAL MASSES:
[list order = Y]
Cleanse the skin over the lesion with an alcohol swab. If local anesthesia is needed, use ethyl chloride spray or 1% lidocaine.
Fix the mass between your fingers to immobilize the mass during the needle passes.
Carefully poise the 23 or 25-guage needle at right angles to the surface of the skin, just touching the point of insertion. Quickly introduce the needle through the skin and advance it into the mass. When the needle has entered the mass, apply strong suction.
Apply negative pressure to the syringe and move the needle back and forth within the mass and in different directions to effect the cutting action of the needle point and retain cells in the needle hollow.
It is not necessary to see visible aspirated material in the hub, but the moment blood or any material appears in the hub, stop aspirating but do not remove the needle from the mass until pressure on the plunger is released; this will allow cells to be contained in the needle and not the syringe. If you do not release the suction, the aspirated material will enter the barrel of the syringe and may result in loss of cellular material as it is difficult to recover material from the syringe.
Place the entire sample directly in CytoLyt® solution by aspirating CytoLyt® solution through the needle into the syringe and then expelling back into the CytoLyt® container several times (see below). Samples from different anatomic sites should be placed in separate containers or slides, labeled appropriately, and submitted with separate requisitions.
Occasionally the aspirated lesion is cystic. In this case, aspirate as much fluid as possible and place the fluid into the CytoLyt® vial. Resample any residual mass following the procedure described above.
[/list] Submit the CytoLyt® sample along with a completed MLabs Surgical / Cytopathology Requisition form, including pertinent patient history. The container should be clearly labeled with the patient’s first and last names as well as a second identifier such as the patient’s birthdate or medical record number. Refrigerate specimens that cannot be delivered immediately and deliver as soon as possible. Do not allow specimens to freeze. See http://www.mlabs.umich.edu/files/pdfs/GL-FNA_Specimen_Guidelines.pdf for FNA Collection Guidelines.

Red Top Tube
Minimum Volume
 

Billing Information

CPT Code
10021-TC Collection, 88173-TC Interpretation
Pro Fee Code
10021 Collection, 88172 Evaluation, 88173 Interpretation
LOINC
 

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Our High Standard

Quality that sets us apart

As the reference laboratory division of Michigan Medicine's Department of Pathology, MLabs shares the institution's commitment to applying established quality principles to clinical laboratory testing. Like other large organizations in complex, consequential fields, we rely on an established approach to monitor quality throughout the testing process.