Days Set Up
Monday - Thursday
Analytic Time

2 - 7 days

MiChart Code
EWSR1/WT1 Translocation (Desmoplastic Small Round Cell Tumor) by PCR
Soft Order Code
DSRCT

Test Updated:

Synonyms

EWS Chimeric Transcript Detection
WT-1 Chimeric Transcript Detection
EWS/WT-1 Desmoplastic Small Round Cell Tumor Translocation Assay
DSRCT TRANSLOCATION ASSAY
DSRCT
MLABEL
DSRCTS
DSRCT TRANSLOCATION ASSAY
EWS/WT1 Translocation
Desmoplastic Small Round Cell Tumor Translocation Detection

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Test Overview

Test Methodology

Reverse transcription followed by Polymerase Chain Reaction (PCR) with detection by capillary electrophoresis.

Test Usage

The histologic and immunophenotypic characteristics of desmoplastic small round cell tumor (DSRCT) can overlap with that of other "small round blue cell" tumors. The recurrent t(11;22)(p13;q12) chromosomal translocation is characteristic of DSRCT and leads to fusion of the EWSR1 gene at 22q12 and the WT1 gene at 11p13. The overwhelming majority of DSRCT cases harbor this specific translocation. Because of its high prevalence, the EWSR1/WT1 fusion transcript is a highly sensitive diagnostic marker for DSRCT. Its detection can aid in the diagnosis of these tumors, especially in those with unusual histologic features or atypical presentations. This test qualitatively detects the EWSR1/WT1 fusion transcript in fresh/frozen or formalin-fixed paraffin embedded tissues.

Reference Range

Interpretive report provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Specimen Requirements

Collection Onsite

For formalin-fixed, paraffin-embedded tissue, a block containing an area with a high percentage of neoplastic cells (for micro-/macro-dissection) is preferred. Unstained, UNBAKED slides (5-8, 10-micron slides; 10-15 if few neoplastic cells are present) with associated H&E stained slide are also acceptable. Decalcified tissue or other fixatives will be accepted and the assay attempted, however these may result in failed testing due to degraded nucleic acid. Both blocks and slides should be stored at room temperature.

Collection Offsite

For formalin-fixed, paraffin-embedded tissue, a block containing an area with a high percentage of neoplastic cells (for micro-/macro-dissection) is preferred. Unstained, UNBAKED slides (5-8, 10-micron slides; 10-15 if few neoplastic cells are present) with associated H&E stained slide are also acceptable. Decalcified tissue or other fixatives will be accepted and the assay attempted, however these may result in failed testing due to degraded nucleic acid. Both blocks and slides should be stored at room temperature.

Normal Volume
Formalin-fixed, paraffin-embedded tissue. Extracted RNA is also acceptable if extracted in a CLIA certified laboratory.
Minimum Volume
 

Billing Information

CPT Code
81401
Pro Fee Code
 
LOINC
 

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Our High Standard

Quality that sets us apart

As the reference laboratory division of Michigan Medicine's Department of Pathology, MLabs shares the institution's commitment to applying established quality principles to clinical laboratory testing. Like other large organizations in complex, consequential fields, we rely on an established approach to monitor quality throughout the testing process.