Days Set Up
Monday - Thursday
Analytic Time

2 - 7 days

MiChart Code
EWSR1/FLI1 and EWSR1/ERG Translocations (Ewing Sarcoma) by PCR
Soft Order Code
EWING

Test Updated:

Synonyms

EWS Chimeric Transcript Detection
EWINGS TRANSLOCATION ASSAY
ERG Chimeric Transcript Detection
FLI1 Chimeric Transcript Detection
EWINGS
EWING
MLABEL
EWINGSS
EWINGS TRANSLOCATION ASSAY
EWS/FLI-1 Translocation
EWS/ERG Translocation
Ewing's Sarcoma
Ewing Sarcoma Translocation Detection

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Test Overview

Test Methodology

Reverse transcription followed by Polymerase Chain Reaction (PCR) with detection by capillary electrophoresis.

Test Usage

Ewing sarcoma (ES) is a member of the "small round blue cell" tumor group, and must be differentiated from other morphologically similar tumors. ES is characterized by recurrent chromosomal rearrangements involving the EWSR1 gene. The great majority are the reciprocal translocation t(11;22)(q24;q12), which occurs in approximately 90% of ES cases. This translocation joins the EWSR1 gene on chromosome 22 and the FLI1 gene on chromosome 11, leading to expression of EWSR1/FLI1 fusion transcripts. Alternate translocations involving EWSR1 occur with lower frequency in ES. Of these, the t(21;22)(q22;q12) translocation is the most common, with an incidence of approximately 5-10%. This rearrangement leads to fusion of the EWSR1 and ERG genes, and is also characteristic of ES. This test qualitatively detects the EWSR1/FLI1 and EWSR1/ERG fusion transcripts in fresh/frozen or formalin-fixed paraffin embedded tissues. Testing can aid in the differential diagnosis of ES.

Reference Range

Interpretive report provided. The specific fusion transcript type is reported with a positive test result.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Specimen Requirements

Collection Onsite

A formalin-fixed, paraffin-embedded tissue block containing an area with a high percentage of neoplastic cells (for micro-/macro-dissection) is preferred. Unstained, UNBAKED slides (5-8, 10-micron slides; 10-15 if few neoplastic cells are present) with associated H&E stained slide are also acceptable. Decalcified tissue or other fixatives will be accepted and the assay attempted, however these may result in failed testing due to degraded nucleic acid. Both blocks and slides should be stored at room temperature.

Collection Offsite

A formalin-fixed, paraffin-embedded tissue block containing an area with a high percentage of neoplastic cells (for micro-/macro-dissection) is preferred. Unstained, UNBAKED slides (5-8, 10-micron slides; 10-15 if few neoplastic cells are present) with associated H&E stained slide are also acceptable. Decalcified tissue or other fixatives will be accepted and the assay attempted, however these may result in failed testing due to degraded nucleic acid. Both blocks and slides should be stored at room temperature.

Normal Volume
Formalin-fixed, paraffin-embedded tissue. Extracted RNA is also acceptable if extracted in a CLIA certified laboratory.
Minimum Volume
 

Billing Information

CPT Code
81401 x2
Pro Fee Code
 
LOINC
 

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Our High Standard

Quality that sets us apart

As the reference laboratory division of Michigan Medicine's Department of Pathology, MLabs shares the institution's commitment to applying established quality principles to clinical laboratory testing. Like other large organizations in complex, consequential fields, we rely on an established approach to monitor quality throughout the testing process.