Days Set Up
Monday - Thursday
Analytic Time

2 - 7 days

MiChart Code
EWSR1/ATF1 Translocation (Clear Cell Sarcoma) by PCR
Soft Order Code
CCS

Test Updated:

Synonyms

MLABEL
CCS
CLEAR CELL SARCOMA ASSAY
t(12;22)(q13;q12) Translocation Detection
Clear Cell Sarcoma Translocation Detection

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Test Overview

Test Methodology

Reverse Transcription Polymerase Chain Reaction (PCR) with capillary electrophoresis

Test Usage

Greater than 90% of clear cell sarcomas harbor the reciprocal chromosomal translocation t(12;22)(q13;q12). This rearrangement joins the EWSR1 and ATF1 genes and leads to expression of EWSR1/ATF1 fusion transcripts. This test detects three EWSR1/ATF1 fusion transcript types: Type 1 (EWSR1 exon 8/ATF1 exon 4), Type 2 (EWSR1 exon 7/ATF1 exon 5), and Type 3 (EWSR1 exon 10/ATF1 exon 5) which collectively account for almost all t(12;22)-bearing clear cell sarcoma cases. Testing for EWSR1/ATF1 fusion is a useful diagnostic adjunct in the differential diagnosis of clear cell sarcoma, since malignant melanoma is not associated with this chimeric transcript.

Reference Range

Interpretive report provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Specimen Requirements

Collection Onsite

A formalin-fixed, paraffin-embedded tissue block containing an area with a high percentage of neoplastic cells (for micro-/macro-dissection) is preferred. Unstained, UNBAKED slides (5-8, 10-micron slides; 10-15 if few neoplastic cells are present) with associated H&E stained slide are also acceptable. Decalcified tissue or other fixatives will be accepted and the assay attempted, however these may result in failed testing due to degraded nucleic acid. Both blocks and slides should be stored at room temperature.

Collection Offsite

A formalin-fixed, paraffin-embedded tissue block containing an area with a high percentage of neoplastic cells (for micro-/macro-dissection) is preferred. Unstained, UNBAKED slides (5-8, 10-micron slides; 10-15 if few neoplastic cells are present) with associated H&E stained slide are also acceptable. Decalcified tissue or other fixatives will be accepted and the assay attempted, however these may result in failed testing due to degraded nucleic acid. Both blocks and slides should be stored at room temperature.

Normal Volume
Formalin-fixed, paraffin-embedded tissue. Extracted RNA is also acceptable if extracted in a CLIA certified laboratory.
Minimum Volume
 

Billing Information

CPT Code
81401
Pro Fee Code
 
LOINC
 

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Our High Standard

Quality that sets us apart

As the reference laboratory division of Michigan Medicine's Department of Pathology, MLabs shares the institution's commitment to applying established quality principles to clinical laboratory testing. Like other large organizations in complex, consequential fields, we rely on an established approach to monitor quality throughout the testing process.