Days Set Up
Daily, 24 hours
Analytic Time

4 hours

MiChart Code
Soft Order Code

Test Updated:


Estradiol (E2)
Estradiol-17 Beta (E2)
Estradiol, Serum

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Test Overview

Test Methodology

Chemiluminescent Immunoassay (CLIA)

Test Usage

Assessment of hypothalmic and pituitary function; to assess ovarian failure and follicular maturation. Amenorrhea work-up: low result, if gonadotropins high, may indicate ovarian failure, if gonadotropins and estradiol low, consider hypothalamic or pituitary disorder. In males E2 measurements may be helpful in the assessment of unexplained gynecomastia. In females E2 measurements are frequently utilized to document hypoestrogenism in cases of delayed puberty, primary and secondary amenorrhea, and menopause. Gonadotropin measurements must be utilized to localize the cause of hypoestrogenism to the ovaries or to a hypothalamic-pituitary source (Perganol(R) induction). E2 concentrations exceeding 1000 pg/ml are usually observed only during pregnancy or in rare cases of estrogen secreting.

Reference Range

Adult male: 6-44 pg/ml; Adult female: early follicular 10-50 pg/ml; late follicular 60-200 pg/ml; midcycle 120-375 pg/ml; luteal phase 50-260 pg/ml; postmenopausal <20 pg/ml.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Specimen Requirements

Collection Onsite

Collect specimen in SST tube. Test is not useful on pregnant women.

Collection Offsite

Collect specimen in SST tube. Centrifuge, aliquot serum into a plastic vial and refrigerate. Test is not useful on pregnant women.

Yellow Top Tube
Normal Volume
0.50 mL serum
Minimum Volume
0.25 mL serum

Billing Information

CPT Code
Pro Fee Code

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Our High Standard

Quality that sets us apart

As the reference laboratory division of Michigan Medicine's Department of Pathology, MLabs shares the institution's commitment to applying established quality principles to clinical laboratory testing. Like other large organizations in complex, consequential fields, we rely on an established approach to monitor quality throughout the testing process.