Test Overview
Test Methodology

Multiplex Flow Immunoassay

Test Usage

Useful diagnostic and prognostic information can be gained in ANA positive patients by knowing the specificity of the autoantibodies that are detected. Ideally, collagen vascular disease diagnosis is based on a combination of clinical symptoms and laboratory testing. It is not cost effective to follow up every positive ANA with assays for all 10 possible detectable antibody specificities. MLabs promotes a more cost effective strategy an ENA 5 Antibody Panel (includes SS-A, SS-B, Sm, RNP, and SmRNP) (order code ENA5). These antibodies will account for the vast majority of significant positive results. Other ENA antigens such as Scl-70, Centromere B, Jo-1, and Ribosomal P should not be routinely screened for, but ordered as individual tests as the differential diagnosis dictates. The laboratory recommends either anti-dsDNA (order code ADNA) or crithidia DNAIFA (order code DNAIF) to detect and quantify anti-dsDNA.

Reference Range *

Negative

Test Details
Days Set Up
Monday - Friday
Analytic Time

1 day

Soft Order Code
ENA7
MiChart Code
Extractable Nuclear Antibody Panel (7)
Laboratory
Chemical Pathology
Section
Immunopathology
Specimen Requirements
Collection Instructions

Collect specimen in SST tube. Centrifuge, aliquot serum into a plastic vial and refrigerate. Please indicate panel or specific antibody testing to be performed.

Alternate Specimen
Red top tube.
Yellow Top Tube
Normal Volume
0.5 mL serum
Minimum Volume
0.25 mL serum
Additional Information

See Extractable Nuclear Antibody Testing.available online at http://www.pathology.med.umich.edu/handbook/Tables/ANA-ENA-08.pdf. ENA5 Panel includes SS-A (Ro), SS-B (La), Smith, SmRNP, and RNP. ENA7 Panel includes SS-A (Ro), SS-B (La), Smith, SmRNP, RNP, Jo-1, and Scleroderma (Scl-70). ENA10 Panel includes SS-A (Ro), SS-B (La), Smith, SmRNP, RNP, Jo-1, Scleroderma (Scl-70), Centromere B, Chromatin, and Ribosomal P. The following antibodies may also be ordered individually: Centromere B (CENT B), Jo-1 (JO-1), Ribosomal P (RIBO P), RNP (RNP), Scleroderma (SCL-70), Smith (SMITH), SS-A (SS-A), and SS-B (SS-B).

Billing
CPT Code
86235 EACH ANTIBODY
Fee Code
20572 ENA5, 20573 ENA7, KA002 ENA10, 32162 RIBO P, 30941 CENT B, 22176 SCL-70, 31528 JO-1
LOINC
Z32-3 ENA5, Z1002-5 ENA7, 8076-2 JO-1, 8092-9 SCL-70, 16137-2 CENT B, Z1001-7 CHROM, 5130-0 DSDNA, 31592-9 RIBO P
NY State Approved
No