Test Overview
Test Methodology

Hexokinase, NADH Endpoint

Test Usage

Determine glucose level, monitor insulin therapy, evaluate carbohydrate metabolism.

Reference Range *

Fasting: <1 year: 40 - 120 mg/dL, 1-4 years: 50 - 130 mg/dL, 5-11 years: 50 - 135 mg/dL, 12-150 years: 73 - 100 mg/dL. 1 Hr Postprandial: 50 gm load: <135 mg/dL, 100 gm load: <190 mg/dL. 2 Hr Postprandial: (75 gm load): Normal <140 mg/dL, Impaired 140 - 200 mg/dL, Provisional Diagnosis >200 mg/dL.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Daily, 24 hours
Analytic Time

2 hours

Soft Order Code
GLUC
Laboratory
Chemical Pathology
Section
Automation
STAT Availability

STAT requests for this test will be performed on a STAT basis (supervisory staff approval is not required).

Specimen Requirements
Collection Instructions

Collect specimen in an SST tube depending on the measurement requested (random, fasting, 1 hour postprandial or 2 hour postprandial). Centrifuge within 4 hours of collection, aliquot serum into a plastic vial and refrigerate. If specimen cannot be centrifuged at client site, please arrange for delivery to MLabs within 3 hours of specimen collection.

Special Handling

If fasting specimen desired, patient should be fasting prior to collection. See Additional Information for GLUC1PP and GLUC2PP (1 or 2 hour posprandial) collection.

Fasting Specimen
12 hour fasting specimen is recommended; 8 hour fasting specimen is acceptable.
Yellow Top Tube
Normal Volume
0.5 mL serum
Minimum Volume
0.25 mL serum
Additional Information

1 hour Postprandial Glucose (GLUC1PP; 50gm load; one sample collection): Administer 50 gm Glucola. Instruct patient to finish drinking the Glucola within 5 minutes of the time he or she starts drinking and not to eat anything except a few sips of water after finishing the Glucola. Collect blood in an SST tube exactly 1 hour after the patient finished drinking the Glucola. Centrifuge, aliquot serum into plastic vial and refrigerate. Label vial appropriately (1 HR PP).

2 Hour Postprandial Glucose (GLUC2PP; 75gm load; one sample collection): Administer 75 gm Glucola. Instruct patient to finish drinking the Glucola within 5 minutes of the time he or she starts drinking and not to eat anything except a few sips of water after finishing the Glucola. Collect blood in an SST tube exactly 2 hours after the patient finished drinking the Glucola. Centrifuge, aliquot serum into plastic vial and refrigerate. Label vial appropriately (2 HR PP).

Billing
CPT Code
82947 GLUC, 82950 GLUC1PP/GLUC2PP
Fee Code
20502 GLUC, 22141 GLUC1PP/GLUC2PP
LOINC
2345-7
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