Routine testing is performed by the complement fixation method. Immunodiffusion methodology will be used to resolve questions of cross-reactivity, if the specimen has anti-complementary antibodies or if specifically requested in suspected cases of blastomycosis.
To obtain serologic evidence of an immune response (antibody production) to Histoplasma capsulatum, Blastomyces dermatitidis, or Coccidioides immitis.
Negative. A four-fold or greater rise in titer between acute and convalescent paired specimens, collected 14-21 days apart is consistent with the diagnosis of recent infection. In the presence of high titer against one fungal pathogen, other low level antibody titers may represent a cross-reaction and are not considered significant.
Test sent to Michigan Department of Health & Human Services (MDHHS); although there is no charge for testing, MLabs charges a handling fee for processing and forwarding specimens to MDHHS.