Multiplex Flow Immunoassay
The ANA screen test detects autoantibodies to 11 clinically relevant antibodies (dsDNA, SS-A, SS-B, Sm, RNP, SmRNP, Scl-70, Jo-1, Centromere B, Ribosomal P, and Chromatin). A negative result means that the patient’s serum shows no reactivity for these common antibodies associated with connective tissue diseases. See also: Extractable Nuclear Antibodies.
Collect specimen in SST tube. Centrifuge, aliquot serum into a plastic vial and refrigerate. Please note if ANA by Multiplexed Immunoassay with Reflex to IFA Titer and Pattern is desired (ANA2).
The ANA by Multiplex Flow Immunoassay has a lower positive rate in patients without connective tissue diseases and eliminates some of the very weak positives (titer 1:80) often seen by IFA that have no detectable specific antibodies. If ANA by Multiplexed Immunoassay with Reflex to IFA Titer and Pattern (ANA2) is requested and the screen is positive, a titer will be performed at an additional charge. If the Hep-2 ANA titer is 1:80 or greater with a homogeneous, speckled, nucleolar, or atypical nuclear matrix pattern in children less than 18 years of age, the more specific tissue ANA will be performed at an additional charge. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated.