DNA Amplification by Strand Displacement Amplification
Detection of Neisseria gonorrhoeae in male or female urogenital specimens.
* Reference ranges may change over time. Please refer to the original patient report when evaluating results.
Adequate specimen collection is important. Mucoid specimens may cause inhibition of the assay. Some feminine sprays, powders, spermicidal agents, and lubricants may interfere with the assay and should not be used prior to specimen collection. A negative result does not exclude the possibility of infection. Predictive value of test will vary depending on disease prevalence. Nucleic acids may persist for up to 4 weeks following appropriate antimicrobial therapy.
Acceptable specimens include endocervix, vagina, and urethra. A specimen collected during menses is acceptable. A single swab from a urogenital specimen can be used for both Chlamydia trachomatis and Neisseria gonorrhoeae DNA amplification. The following specimens are unacceptable: white shaft cleaning swabs, wooden swabs, or Calgiswabs.
[list order = Y]
ENDOCERVICAL SPECIMEN: Clean excess mucus from the cervical os and surrounding mucosa using the white shaft cleaning swab or other cleaning swab of choice. Discard cleaning swab; do not use white shaft cleaning swab to collect sample. Insert the small PINK swab from the collection kit 1 to 1.5 cm into the endocervical canal, rotate the swab clockwise for 15 to 30 seconds, and draw the swab back carefully avoiding any contact with the vaginal mucosa. Insert the swab all the way into the transport media immediately after specimen collection and bend the shaft of swab against the tube to break shaft; it is scored to break easily if the swab is inserted completely into transport. TIGHTEN LID OF TRANSPORT TUBE SECURELY TO PREVENT LOSS OF FLUID.
URETHRAL SPECIMEN: Insert the small BLUE swab from the collection kit 2 to 4 cm into the urethra, rotate clockwise for 3 to 5 seconds and withdraw. Insert the swab all the way into the transport media immediately after specimen collection and bend the shaft of swab against the tube to break shaft; it is scored to break easily if the swab is inserted completely into transport. TIGHTEN LID OF TRANSPORT TUBE SECURELY TO PREVENT LOSS OF FLUID.
VAGINAL SPECIMEN: Patient collected vaginal swab specimens are an option for screening women when a pelvic exam is not otherwise indicated; it is not for home use. Ensure the patient washes hands with soap and water. The patient should collect the sample by holding the swab with one hand and spreading skin outside the vagina. She should then insert the tip of the swab into the vaginal opening no more than 2 inches, gently rotate for 10 to 15 seconds, withdraw the swab and place the swab in the transport tube and cap securely. After collection, the patient should wash her hands and return the tube to the clinician.
A single swab can be used for Chlamydia trachomatis, Neisseria gonorrhoeae, and/or Trichomonas vaginalis DNA Amplification. Transport tubes will be held for 1 week. Additional testing for Chlamydia trachomatis or Trichomonas vaginalis may be requested by contacting the MLabs Client Services Center.