Test Overview
Direct immunofluorescence using flow cytometric techniques.
Confirmation of diagnosis and immunophenotype of plasma cell disorders. See http://www.pathology.med.umich.edu/handbook/Tables/Flow_Cytometry_Panel… for all Flow Cytometry Panels.
Pathologist interpretation of results provided.
Requires an adequate number of viable cells for analysis. When cell counts drop below 5 cells/cmm and sample volume is 1 ml or less, immunophenotypic analysis may not be successful.
Test Details
8 - 48 hours
- Plasmacytoma
- Plasma Cell Dyscrasia
- Immunophenotypic Plasma Cell Panel
Specimen Requirements
The following information is required on a Hematopathology Consult requisition:
<ul><li><li>
Relevant clinical history
Clinical or morphological findings and suspicions
Specimen source
Date and time of collection
Phone number and after hours pager number for ordering physician
</ul>
<b>BLOOD: </b>
<ul><li>
Collect in a 7-10 ml green sodium heparin Vacutainer® tube and transport at room temperature within 12 hours of collection
Include a copy of the patient's concurrently drawn White Blood Cell and Platelet counts, peripheral blood differential and an unstained peripheral blood smear, OR, concurrently collect a lavender EDTA Vacutainer® tube for a CBCD to be performed upon receipt of the specimen.
</ul>
<b>BONE MARROW ASPIRATE: </b>
<ul><li>
Add 1 - 2 ml of first pull bone marrow aspirate to a green top sodium heparin Vacutainer® tube.<li>Send intact specimen at room temperature within 12 hours of collection.<li>
Include 4 unstained aspirate smears
Include an unstained peripheral blood smear
Include a copy of the patient's most recent White Blood Cell and platelet counts, and peripheral blood differential, OR, concurrently collect a lavender EDTA Vacutainer® tube for a CBCD to be performed upon receipt of the specimen.
</ul>
<b>BODY FLUID and CSF: </b>
<ul><li>
Add body fluid (e.g., CSF, pleural, peritoneal) to a clean, leak proof sterile container and send at room temperature within 12 hours of collection.<li>
Include a a copy of the body fluid cell count and differential.<li>
An original cytospin preparation (preferably unstained) should be included when possible with CSF specimens for correlative morphological evaluation.<li>
</ul>
<b>BONE MARROW CORE BIOPSY AND FNA: </b>
<ul><li>
Submerge in RPMI tissue culture medium (for optimal cell viability) or sterile saline and transport at room temperature within 12 hours of collection.
</ul>
<b>FRESH TISSUE: </b>
<ul><li>
Mince and submerge in RPMI tissue culture medium (for optimal cell viability) or sterile saline, refrigerate, and transport refrigerated within 12 hours of collection.<li>
</ul>
<b>BLOOD: </b> 7-10 ml
<b>BONE MARROW ASPIRATE: </b> 1-2 ml
<b>BODY FLUIDS: </b> 20-50 ml
<b>CSF: </b> 5-10 ml
</ul>
<b>BLOOD: </b> 1 ml
<b>BONE MARROW ASPIRATE: </b> 1 ml
<b>BODY FLUIDS: </b> 5 ml*
<b>CSF: </b> 1 ml*
</ul>
* The volume of Body Fluids and CSF required is dependent on the cellularity of the specimen.
Additional Information
Test includes pathologist interpretation of results billed as a separate additional charge. This test is not available without interpretation. Additonal markers will be run if clinically indicated. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. For consultation contact the MLabs Client Services Center or Flow Cytometry Laboratory at 76-39420.