Test Overview
Test Methodology

EIA

Test Usage

To determine presence or absence of C. difficile antigen and toxins A and B in fecal specimens.

Reference Range *

Negative.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

Per Michigan Medicine Clostridium difficile Infection Guidelines testing is rarely indicated or recommended for infants less than 12 months and consultation with Pediatric ID is recommended.

Test Details
Days Set Up
Daily (reflex PCR run once per day)
Analytic Time

EIA: 8 hours; reflex PCR: 1 day

Soft Order Code
CDIFF
MiChart Code
Clostridium difficile (C. diff) by EIA
Synonyms
  • Antibiotic Associated Colitis (AAC)
  • Colitis, Clostridium difficile
  • Pseudomembranous Colitis Toxin Assay
  • PCRCD
  • CLOST. DIFFICILE TOXIN B, PCR
  • CDIFF
  • Clostridium difficile Antigen
  • Clostridium difficile Toxin
  • CDTOX
  • CLOSTRIDIUM DIFFICILE TOXIN
  • CLOSTRIDIUM DIFFICILE
  • GDH Screen
  • GDH Antigen
Laboratory
Microbiology
Section
Microbiology
Specimen Requirements
Collection

Test should be requested only on patients suspected to have antibiotic associated colitis (AAC). Collect at least 5 mL of fresh stool and place in a sterile screw-capped container with no preservative. Formed stools are not acceptable. Refrigerate and send specimen within 48 hours of collection. Indicate specimen source, collection date/time, current antibiotic therapy, and clinical diagnosis. Collect no more than one specimen in a 24 hour period; after two negative tests, wait 10 days before sending another specimen; after one positive test, wait 14 days before sending another specimen. If an unacceptable specimen is received, the client will be notified before disposal of the original specimen. See MLabs Stool Specimen Collection Instructions available online at http://mlabs.umich.edu/files/pdfs/GL-Stool_Collection_Guidelines.pdf for patient instructions.

Rejection Criteria
Specimens retrieved from a diaper or contaminated with urine are not acceptable.
Contraindications
Test should be requested only on patients suspected to have antibiotic associated colitis (AAC).
Normal Volume
5 mL liquid stool
Minimum Volume
5 mL liquid stool (specimens retrieved from a diaper or contaminated with urine are not acceptable)
Additional Information

If EIA for C. difficile is inconclusive, confirmation testing for C. difficile Toxin DNA by PCR (PCRCD) will be performed at an additional charge. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated.

Billing
CPT Code
87449
Fee Code
32397
Reflex CPT
87798 PCRCD
Reflex Fee Code
32277 PCRCD
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