Test Overview
Test Methodology

Polymerase Chain Reaction (PCR) followed by sequencing analysis to detect CEBPA mutation if present

Test Usage

Mutations within the CEBPA gene occur in approximately 5-10% of patients with newly diagnosed acute myeloid leukemia (AML), and are most frequent in cases with a normal karyotype. The presence of CEBPA mutations has been associated with a more favorable clinical outcome. Two types of CEBPA mutations are most frequently observed: N-terminal frameshift mutations that lead to truncation of the full-length protein, and C-terminal bZIP domain region mutations that are in-frame insertions or deletions. Both mutation types usually occur simultaneously (double mutations), although up to a third of cases may exhibit only one type (single mutation). This DNA sequencing test will detect single and double mutations within the entire CEBPA coding region from blood or bone marrow specimens. Testing for CEBPA mutations may aid in prediction of clinical outcome.

Reference Range *

Interpretive report provided.

Test Limitations

The limit of detection of this assay is 40% mutation-bearing cells. Mutations may not be detected in samples with a neoplastic burden below this level.

Test Details
Days Set Up
Monday - Thursday
Analytic Time

3 - 10 days

Soft Order Code
CEBPA
MiChart Code
CEBPA Mutation
Synonyms
  • MLABEL
  • CEBPA
  • CEBPA Mutation Detection
  • C/EBP Alpha Mutation Detection
Laboratory
Molecular Diagnostics
Section
Molecular Diagnostics
Specimen Requirements
Collection Instructions

Collect blood or bone marrow in a lavender top tube. Refrigerate and send intact blood or bone marrow specimen within 48 hours of collection. Fresh tissue (preferably 0.5cm3, sent in RPMI) and fresh aspirates or body fluids are acceptable. Refrigerate and send, preferably within 24 hours. Frozen tissue specimens – preferably frozen with 1 hour of collection – may also be sent frozen on dry ice. Fresh cell suspensions in RPMI should be refrigerated and sent, preferably within 48 hours. Frozen cell suspensions – preferably frozen with 1 hour of collection – may also be sent frozen on dry ice. Fixed cytogenetic cell suspensions or pellets in Carnoy’s fixative. Refrigerate and send.

Alternate Specimen
The preferred specimen is whole blood or bone marrow collected in EDTA (lavender top). However, ACD (yellow top) and heparin (green top) may also be accepted. Previously extracted DNA may also be accepted.
Rejection Criteria
Formalin-fixed, paraffin-embedded specimens are not acceptable.
Lavender Top Tube
Normal Volume
5 mL EDTA (lavender) whole blood; 3 mL EDTA (lavender) bone marrow; Fresh/frozen tissue and fresh aspirates or body fluids; Fresh/frozen cells in RPMI; Fixed cytogenetic cell suspensions or pellets in Carnoy’s fixative. Extracted DNA is also acceptable if extracted in a CLIA certified laboratory.
Additional Information

By ordering this test the clinician acknowledges that informed consent has been obtained from the patient as required by applicable state or federal laws and the ordering clinician has authorization from the patient permitting MLabs to report the test results to the ordering clinician. Test includes pathologist interpretation of results billed as a separate additional charge. This test is not available without interpretation.

Billing
CPT Code
81218
Fee Code
NA055
Pro Fee CPT
G0452-26
NY State Approved
No