Days Set Up
Daily, 24 hrs
Analytic Time

Preliminary results are reported in 24 hours. Negative cultures are reported in 5 days. The health care provider will be notified of initial positive cultures and gram stain results. Verigene Blood Culture Nucleic Acid Tests will be run on the first positive set of bottles in an episode of bacteremia. Results will typically be available 3-6 hours after the time of gram stain notification.

Soft Order Code
BLD/BLDAE/BLDAN/BLDOT/ BLDC

Test Updated:

Synonyms

BLD
BLDAE
BLDAN
ADULT BLOOD CULTURE
BLOOD CULTURE (AER)
BLOOD CULTURE (ANA)
Anaerobe Culture, Blood
Blood Culture, Brucella
Blood Culture, Candida
Blood Culture, Yeast
Brucella Blood Culture
Candida Blood Culture
Culture, Blood, Bacteria
Culture, Blood-Bacteria & Yeast
SBE, Blood Culture
Yeast Blood Culture
Anaerobe Culture, Blood
Blood Culture, Brucella
Blood Culture, Candida
Blood Culture, Yeast
Brucella Blood Culture
Candida Blood Culture
Culture, Blood, Bacteria
Culture, Blood-Bacteria & Yeast
SBE, Blood Culture
Yeast Blood Culture
Anaerobe Culture, Blood
Blood Culture, Brucella
Blood Culture, Candida
Blood Culture, Yeast
Brucella Blood Culture
Candida Blood Culture
Culture, Blood, Bacteria
Culture, Blood-Bacteria & Yeast
SBE, Blood Culture
Yeast Blood Culture
Anaerobe Culture, Blood
Blood Culture, Brucella
Blood Culture, Candida
Blood Culture, Yeast
Brucella Blood Culture
Candida Blood Culture
Culture, Blood, Bacteria
Culture, Blood-Bacteria & Yeast
SBE, Blood Culture
Yeast Blood Culture
BLDOTH
ADULT BLOOD CULTURE
BLOOD CULTURE
BLOOD CULTURE
BLOOD CULTURE
Verigene Gram-Negative Blood Culture Nucleic Acid Test (BC-GN)
Verigene Gram-Positive Blood Culture Nucleic Acid Test (BC-GP)

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Test Overview

Test Methodology

BD Bactec FX Blood Culture System; Verigene® Gram-Positive Blood Culture Nucleic Acid Test (BC-GP); Verigene® Gram-Negative Blood Culture Nucleic Acid Test (BC-GN)

Test Usage

Isolate and identify bacteria or yeast causing bacteremia or fungemia.

Reference Range

No growth

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Specimen Requirements

Collection

Two blood culture sets (if BLDAE ordered, only the aerobic bottle is needed) from different anatomic sites should be submitted to detect suspected bacteremia or fungemia. Draw blood before initiating antibiotic therapy if possible. Invert BD BACTEC bottles after each collection. For optimum recovery, send immediately to Microbiology or store BD BACTEC bottles at room temperature and send within 18-24 hours. Do not refrigerate. A maximum of 3 blood culture sets will be accepted within a 24-hour period. If an unacceptable specimen is received, the client will be notified before disposal of the original specimen.

Remove outer cap and cleanse bottle tops with alcohol. Clean venipuncture site with soap and water if soiled. Wipe with 70% alcohol and allow to dry. Place swab containing an iodoform solution on the venipuncture site and scrub vigorously for 30 seconds. Allow iodoform solution to remain intact on the skin for 30 seconds before performing the venipuncture. Do not palpate venipuncture site after preparation. If the patient is sensitive to iodine, alcohol alone may be used. Draw Aerobic (blue top) first then draw Anaerobic (purple top). Draw 8-10 mL of blood (minimum 1-3 mL) into each bottle using a butterfly needle. Do not overfill. Label each bottle individually with patient's name, registration number, and anatomic site of blood draw. Place label vertically on the bottle, do not put label on bottom of bottles or across two bottles since this interferes with loading bottles on the instrument. See https://www.pathology.med.umich.edu/static/apps/handbook/Tables/Blood%2… for specimen collection tip sheet.

Additional Instructions:
• Fever of undetermined origin: obtain 2 separate blood cultures initially and 2 more 24-36 hours later, collected 1 hour before the expected temperature elevation.
• Acute endocarditis: obtain 3 separate blood cultures during the first 1-2 hours of evaluation and begin therapy.
• Subacute endocarditis: obtain 3 blood cultures on day 1. If all are negative 24 hours later, obtain 3 more.
• Filamentous fungus (e.g., Histoplasma or Aspergillus sp.) suspected: send isolator tube for fungus blood culture (Fungus Culture, Blood).
• Mycobacterium sp. suspected: send an isolator tube for an AFB blood culture (AFB Culture, Blood).

Normal Volume
Adult 8-10 mL per bottle.
Pediatric: (aerobic only) 1-3 mL in accordance with physician recommendation for each patient.
DO NOT OVERFILL.
Minimum Volume
0.5 mL per bottle

Billing Information

CPT Code
87040
Pro Fee Code
 
LOINC
 

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Our High Standard

Quality that sets us apart

As the reference laboratory division of Michigan Medicine's Department of Pathology, MLabs shares the institution's commitment to applying established quality principles to clinical laboratory testing. Like other large organizations in complex, consequential fields, we rely on an established approach to monitor quality throughout the testing process.