Test Overview

Test Methodology

Screen: Indirect immunofluorescence (IFA), HEp-2000 substrate. If positive reflex to multiplex immunobead and anti-ds-DNA (EIA) assays (and tissue ANA where indicated)

Test Usage

Initial Screening test for wide variety of autoimmune diseases including SLE, scleroderma, mixed connective issue disease and others. HEp-2000 cell substrate indirect immunofluorescence pattern and titer will be reported on the positive screens.
A positive screen will reflex to an additional testing cascade which is designed to identify the specificity of antinuclear antibodies observed in the screening assay. See Testing: adult algorithm
A positive screen in a child with concordant clinical findings suggests the possibility of SLE or juvenile rheumatoid arthritis/juvenile chronic arthritis. A positive ANA HEp-2000 IFA screen will reflex to a pediatric specific algorithm. See testing: pediatric algorithm

Reference Range*

ANA Screen: Negative, Titer: <1:80. See test specific handbook entries for reflexed testing (ADNA and ENA10) reference ranges.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details

Days Test Performed

Monday - Friday

Analytic Time

24 - 48 hours

Soft Order Code


MiChart Code

ANA Screening Algorithm (IFA HEp-2000 Substrate reflex to ENA Sub-serology


Chemical Pathology

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Specimen Requirements

Offsite Collection Instructions

Collect specimen in SST tube. Centrifuge, aliquot serum into a plastic vial and refrigerate.

Rejection Criteria

Fluid and plasma specimens are not acceptable for NAB testing.


Yellow Top Tube

Normal Volume

2.5 mL serum

Minimum Volume

1.5 mL serum

Billing Information

Fee Codes

CPT Code


Reflex Fee Code

30914 NAB Titer, 30950 ANA, OA003 ADNA, KA002 ENA10, 21903 ANA Tissue (pediatric), 21936 ANA Tissue Titer (pediatric)


42254-3 NAB, 5048-4 NAB Titer, 8061-4 ANA, 33800-4 ADNA, Z1002-5, 16137-2, Z1001-7,31592-9 ENA10

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