Days Set Up
Monday - Friday
Analytic Time

24 - 48 hours

MiChart Code
ANA Screening Algorithm (IFA HEp-2000 Substrate reflex to ENA Sub-serology
Soft Order Code

Test Updated:


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Test Overview

Test Methodology

Screen: Indirect immunofluorescence (IFA), HEp-2000 substrate. If positive reflex to multiplex immunobead and anti-ds-DNA (EIA) assays (and tissue ANA where indicated)

Test Usage

Initial Screening test for wide variety of autoimmune diseases including SLE, scleroderma, mixed connective issue disease and others. HEp-2000 cell substrate indirect immunofluorescence pattern and titer will be reported on the positive screens.
A positive screen will reflex to an additional testing cascade which is designed to identify the specificity of antinuclear antibodies observed in the screening assay. See Testing: adult algorithm
A positive screen in a child with concordant clinical findings suggests the possibility of SLE or juvenile rheumatoid arthritis/juvenile chronic arthritis. A positive ANA HEp-2000 IFA screen will reflex to a pediatric specific algorithm. See testing: pediatric algorithm

Reference Range

ANA Screen: Negative, Titer: <1:80. See test specific handbook entries for reflexed testing (ADNA and ENA10) reference ranges.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Specimen Requirements

Collection Offsite

Collect specimen in SST tube. Centrifuge, aliquot serum into a plastic vial and refrigerate.

Yellow Top Tube
Normal Volume
2.5 mL serum
Minimum Volume
1.5 mL serum

Billing Information

CPT Code
Pro Fee Code
42254-3 NAB, 5048-4 NAB Titer, 8061-4 ANA, 33800-4 ADNA, Z1002-5, 16137-2, Z1001-7,31592-9 ENA10

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Our High Standard

Quality that sets us apart

As the reference laboratory division of Michigan Medicine's Department of Pathology, MLabs shares the institution's commitment to applying established quality principles to clinical laboratory testing. Like other large organizations in complex, consequential fields, we rely on an established approach to monitor quality throughout the testing process.